Bur M E, Greene G L, Press M F
Department of Pathology, University of Chicago, Illinois 60637.
Int J Gynecol Pathol. 1987;6(2):140-51. doi: 10.1097/00004347-198706000-00006.
Monoclonal estrogen receptor (ER) antibody and indirect immunoperoxidase techniques were used to study the immunohistochemical localization of ER in paraffin sections of 20 surgical specimens containing foci of endometriosis. The eutopic endometrium, available in 16 of 20 cases, was dated histologically and stained for ER. Specific nuclear staining was observed in glandular and stromal cells of the endometrium during the proliferative and early secretory phases. Mid and late secretory phase endometria showed only focal staining. Dating of the endometriotic tissues using morphologic criteria revealed that these were in phase with the endometrium in nine of 16 cases. In these cases the ER staining of both the endometriotic tissues and endometrium was similar. The endometriotic tissues could not be dated as either proliferative or secretory in seven cases and stained variably for ER. Diffuse nuclear staining for ER was present in the endometriotic glands of three of these cases in which the endometriotic glands morphologically resembled glands of the basalis contrasting with weak, focal staining in the corresponding eutopic secretory endometria. The majority of endometriotic tissues examined mimicked the cyclic ER expression of the eutopic endometrium and thus appeared to correspond to the morphologic appearance of the endometriotic glands. Immunohistochemical staining with monoclonal estrophilin antibodies may be helpful in supporting the histopathologic diagnosis of endometriosis.
采用单克隆雌激素受体（ER）抗体和间接免疫过氧化物酶技术，研究了20例含有子宫内膜异位症病灶的手术标本石蜡切片中ER的免疫组织化学定位。20例中有16例可获得在位内膜，对其进行组织学分期并进行ER染色。在增殖期和分泌早期，子宫内膜的腺细胞和基质细胞中观察到特异性核染色。分泌中期和晚期的子宫内膜仅显示局灶性染色。根据形态学标准对异位内膜组织进行分期，发现16例中有9例与在位内膜处于同一时期。在这些病例中，异位内膜组织和在位内膜的ER染色相似。7例异位内膜组织无法判定为增殖期或分泌期，ER染色结果各异。其中3例异位内膜腺体呈弥漫性核染色，这些异位内膜腺体在形态上类似于基底层腺体，与之形成对比的是，相应的在位分泌期内膜呈弱阳性、局灶性染色。大多数检测的异位内膜组织模仿了在位内膜的周期性ER表达，因此似乎与异位内膜腺体的形态外观相符。用单克隆雌激素受体结合蛋白抗体进行免疫组织化学染色可能有助于支持子宫内膜异位症的组织病理学诊断。
