Department of Basic Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Islamic Azad University, Tehran, Iran.
Department of Physiology, Faculty of Basic Sciences, Hamadan Branch, Islamic Azad University, Hamadan, Iran.
J Gastrointest Cancer. 2022 Mar;53(1):99-104. doi: 10.1007/s12029-020-00434-8.
This study is aimed to assess the anti-cancer effects of Celecoxib and topotecan against Human Gastric cancer cell line (AGS) in comparison to the control in an in-vitro study.
In this experimental study, Celecoxib and topotecan was prepared at concentrations of 500, 250, 125, 62.5, 31.2, 15.6 and 7.8 mg/ml. The effect of celecoxib and topotecan separately and in mixed form were investigated on AGS and normal HEK cells. To investigate the cell survival, MTT method was used to study the pathway of apoptosis using flowcytometry and Caspase kits based on colorimetric. Finally, one-way ANOVA and t-test were used to analyze the data.
The results of this study indicated that Celecoxib was cytotoxic against AGS and HEK cell lines. The topotecan indicated a significant cytotoxicity against AGS cells and was not toxic against HEK cell line. Our results indicated that Celecoxib and topotecan have synergist effects against AGS and HEK cell lines and were more effective than separate celecoxib or topotecan.
The mixture of clecoxib and topotecan was more effective than celecoxib and topotecan in separate form. Our results indicated that use mixed forms of treatments can cause excellent therapeutic effects and can cause less side effects.
本研究旨在评估塞来昔布和拓扑替康对人胃癌细胞系（AGS）的抗癌作用，并与对照进行体外研究。
在这项实验研究中，塞来昔布和拓扑替康分别制备成浓度为 500、250、125、62.5、31.2、15.6 和 7.8mg/ml。分别研究塞来昔布和拓扑替康单独和混合形式对 AGS 和正常 HEK 细胞的影响。为了研究细胞存活，采用 MTT 法研究细胞凋亡途径，采用流式细胞术和基于比色法的 Caspase 试剂盒。最后，采用单因素方差分析和 t 检验对数据进行分析。
本研究结果表明，塞来昔布对 AGS 和 HEK 细胞系具有细胞毒性。拓扑替康对 AGS 细胞表现出显著的细胞毒性，而对 HEK 细胞系没有毒性。我们的结果表明，塞来昔布和拓扑替康对 AGS 和 HEK 细胞系具有协同作用，且比单独使用塞来昔布或拓扑替康更有效。
塞来昔布和拓扑替康的混合物比单独使用塞来昔布和拓扑替康更有效。我们的结果表明，使用混合形式的治疗可以产生极好的治疗效果，并可以减少副作用。
