College of Pharmacy, Beihua University, Jilin, China.
Department of Hematology, PLA General Hospital, Beijing, China.
J Food Biochem. 2021 Jan;45(1):e13555. doi: 10.1111/jfbc.13555. Epub 2020 Nov 16.
This experiment mainly optimized the extraction technology of Agaricus blazei polypeptide (ABp) and evaluated its protective effect on aging mice. In this study, a novel single component, the M is 3 kD, was isolated and purified from Agaricus blazei. An aging mouse model was established using D-galactose. After the administration of ABp, the contents of total antioxidant capacity (T-AOC), malondialdehyde (MDA), catalase (CAT), and reactive oxygen species were significantly changed. Through immunofluorescence staining, it was observed that ABp can reduce changes in brain tissue. The differential expression of genes was analyzed by RNA-seq. A total of 295 differentially expressed genes were screened out in the ABp group.RT-qPCR verified important genes and showed that the mRNA expression levels of Hsph1, Trim32, HK1, Hnrnpa1, and Grik5 were significantly increased, and those of ApoE, Atp1a3, Stxbp1, and Mapk8ip1 was significantly decreased. Western blotting showed that the protein expression levels of Keap1 and p53 were significantly lower, while the protein expression levels of Nrf2, HO-1, Hsph1, and Trim32 were significantly higher in the ABP group. ABp played an anti-aging role in an aging mouse model. The specific mechanism of action may be related to the regulation of the expression of the Keap1/Nrf2/P53 signaling pathway and related factors. PRACTICAL APPLICATIONS: The research may contribute to the development of ABp as functional foods or dietary supplements for anti-aging in the future.
本实验主要优化了姬松茸多肽（ABp）的提取工艺，并评价了其对衰老小鼠的保护作用。本研究从姬松茸中分离得到一种新型单一成分，分子量为 3kD。采用 D-半乳糖建立衰老小鼠模型。给予 ABp 后，总抗氧化能力（T-AOC）、丙二醛（MDA）、过氧化氢酶（CAT）和活性氧含量的含量均发生显著变化。通过免疫荧光染色观察到 ABp 可减少脑组织的变化。通过 RNA-seq 分析差异表达基因。在 ABp 组中筛选出 295 个差异表达基因。RT-qPCR 验证了重要基因，并显示 Hsph1、Trim32、HK1、Hnrnpa1 和 Grik5 的 mRNA 表达水平显著增加，而 ApoE、Atp1a3、Stxbp1 和 Mapk8ip1 的 mRNA 表达水平显著降低。Western blotting 显示 ABp 组 Keap1 和 p53 的蛋白表达水平显著降低，而 Nrf2、HO-1、Hsph1 和 Trim32 的蛋白表达水平显著升高。ABp 在衰老小鼠模型中发挥抗衰老作用。其具体作用机制可能与 Keap1/Nrf2/P53 信号通路及相关因子的表达调节有关。实际应用：本研究可能有助于开发 ABp 作为未来抗衰老的功能性食品或膳食补充剂。
