Erber W N, Jacobs A, Oscier D G, O'Hea A M, Mason D Y
Department of Haematology, John Radcliffe Hospital, Oxford.
J Clin Pathol. 1987 Nov;40(11):1349-52. doi: 10.1136/jcp.40.11.1349.
The alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical staining technique was used to look for circulating cells of megakaryocyte lineage in peripheral blood smears from 67 cases of myelodysplasia. Small numbers of micromegakaryocytes positive for platelet glycoprotein IIIa were found in 23 cases. These cells superficially resemble small lymphoid cells and are hence difficult or impossible to recognise in conventional Romanowsky stained smears. Circulating micromegakaryocytes were found most commonly in more aggressive types of myelodysplasia (such as refractory anaemia with excess blasts (RAEB) and refractory anaemia with excess blasts in transformation (RAEB-t], and their presence may therefore indicate a poor prognosis. Because of the simplicity of this immunocytochemical labelling technique, it could be of wide use in the initial assessment of patients with myelodysplasia, and possibly for the early detection of acute leukaemic transformation.
采用碱性磷酸酶-抗碱性磷酸酶（APAAP）免疫细胞化学染色技术，对67例骨髓增生异常综合征患者的外周血涂片进行检查，以寻找巨核细胞系的循环细胞。23例患者中发现少量血小板糖蛋白IIIa阳性的微小巨核细胞。这些细胞表面上类似于小淋巴细胞，因此在传统的罗曼诺夫斯基染色涂片中很难或无法识别。循环微小巨核细胞最常见于侵袭性更强的骨髓增生异常综合征类型（如伴有过多原始细胞的难治性贫血（RAEB）和转化中的伴有过多原始细胞的难治性贫血（RAEB-t）），因此它们的存在可能预示预后不良。由于这种免疫细胞化学标记技术操作简便，可广泛应用于骨髓增生异常综合征患者的初步评估，也可能用于急性白血病转化的早期检测。
