Nilsson H, Johansson C, Scheynius A
Department of Clinical Bacteriology, University of Uppsala, Sweden.
J Immunol Methods. 1987 Dec 24;105(2):165-9. doi: 10.1016/0022-1759(87)90262-6.
The possibility of eliminating the class II antigen-expressing and antigen-presenting Langerhans cells from normal human epidermal cell suspensions was investigated. Cell suspensions containing 1.7-2.8% Langerhans cells were prepared by enzyme treatment of human skin obtained at plastic surgery. The cells were incubated with mouse monoclonal antibodies directed against the CD1 antigen present on Langerhans cells and were further incubated with magnetic particles, 4.5 micron in diameter, coated with sheep anti-mouse IgG. The optimal ratio between particles and target cells was found to be 40:1. The rosetted Langerhans cells were removed by a cobalt-samarium magnet. In six experiments immunoperoxidase staining revealed 0-0.1% (mean 0.03%) CD1-reactive Langerhans cells in the depleted cell fraction. The alloantigen presenting capacity of the depleted cell fraction, measured by [3H]thymidine incorporation, was abolished.
研究了从正常人表皮细胞悬液中去除表达II类抗原和呈递抗原的朗格汉斯细胞的可能性。通过酶处理整形手术获取的人皮肤制备出含有1.7%-2.8%朗格汉斯细胞的细胞悬液。将细胞与针对朗格汉斯细胞上存在的CD1抗原的小鼠单克隆抗体孵育，然后再与包被有羊抗小鼠IgG的直径为4.5微米的磁性颗粒孵育。发现颗粒与靶细胞之间的最佳比例为40:1。通过钴钐磁体去除形成玫瑰花结的朗格汉斯细胞。在六个实验中，免疫过氧化物酶染色显示在耗尽细胞组分中CD1反应性朗格汉斯细胞为0%-0.1%（平均0.03%）。通过[3H]胸苷掺入法测定，耗尽细胞组分的同种异体抗原呈递能力被消除。
