对比分析兼性金属耐药菌中间欧文氏菌 BPS-20 和兼性金属耐药菌油菜黄单胞菌 BPS-26 的重金属生物累积和功能基因注释。
A comparative analysis of heavy metal bioaccumulation and functional gene annotation towards multiple metal resistant potential by Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26.
机构信息
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India.
Enzyme Technology and Protein Bioinformatics Laboratory, Department of Microbiology, Maharshi Dayanand University, Rohtak-124001, Haryana, India; School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi 221005, India.
出版信息
Bioresour Technol. 2021 Jan;320(Pt B):124330. doi: 10.1016/j.biortech.2020.124330. Epub 2020 Oct 28.
The present study describes the heavy metal bioaccumulation potential of Ochrobactrum intermedium BPS-20 and Ochrobactrum ciceri BPS-26. A total of 27 isolates were retrieved from the soils of industrial areas and these two were selected based on their maximum metal tolerance. They can resist up to 2400 mg/L and 2000 mg/L of Lead and 850 mg/L and 1200 mg/L of Nickel respectively. The atomic absorption spectroscopic analysis showed considerably good bioaccumulation by O. intermedium BPS-20 (85.34% and 74.87%) and O. ciceri BPS-26 (71.20% and 88.48%) for Lead and Nickel respectively. The growth rate studies also demonstrated no inhibitory effects of heavy metals in the medium. Further the SEM analysis showed the presence of extracellular polymeric substances around bacterial cells. Moreover, the functional gene annotation confirmed the presence of ATPase, ABC, and HoxN/HupN/NixA families of transporters. Thus, both the isolates provide a better solution for the removal of metal pollutants.
本研究描述了中慢生根瘤菌 BPS-20 和慢生小麦根瘤菌 BPS-26 的重金属生物积累潜力。从工业区土壤中总共回收了 27 株分离物，根据它们的最大金属耐受性选择了这两种菌。它们可以分别耐受高达 2400mg/L 和 2000mg/L 的铅以及 850mg/L 和 1200mg/L 的镍。原子吸收光谱分析显示，中慢生根瘤菌 BPS-20 对铅（85.34% 和 74.87%）和镍（71.20% 和 88.48%）的生物积累能力相当好，慢生小麦根瘤菌 BPS-26 也是如此。生长率研究也表明，重金属在培养基中没有抑制作用。进一步的扫描电子显微镜分析显示，细菌细胞周围存在细胞外多聚物物质。此外，功能基因注释证实了存在 ATPase、ABC 和 HoxN/HupN/NixA 转运蛋白家族。因此，这两种分离物都为去除金属污染物提供了更好的解决方案。
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