Department of Experimental Medicine, Section of Medical Physiopathology, Food Science and Endocrinology, "Sapienza" University of Rome, Rome, Italy.
Department of Molecular Medicine, "Sapienza" University of Rome, 00161 Rome, Italy.
Nutrients. 2020 Nov 14;12(11):3506. doi: 10.3390/nu12113506.
Sirtuin 1 (SIRT1) is a sensor of cell energy availability, and with leptin and adiponectin, it regulates metabolic homeostasis. Widely studied in tissues, SIRT1 is under evaluation as a plasmatic marker. We aimed at assessing whether circulating SIRT1 behaves consistently with leptin and adiponectin in conditions of deficiency, excess or normal fat content. Eighty subjects were evaluated: 27 with anorexia nervosa (AN), 26 normal-weight and 27 with obesity. Bloodstream SIRT1, leptin and adiponectin (ELISA), total and trunk fat mass (FM) %, abdominal visceral adipose tissue, liver steatosis and epicardial fat thickness (EFT) were assessed. For each fat store, the coefficient of determination (R) was used to evaluate the prediction capability of SIRT1, leptin and adiponectin. Plasma SIRT1 and adiponectin coherently decreased with the increase of FM, while the opposite occurred with leptin. Mean levels of each analyte were different between groups ( < 0.005). A significant association between plasma variables and FM depots was observed. SIRT1 showed a good predictive strength for FM, particularly in the obesity group, where the best R was recorded for EFT (R = 0.7). Blood SIRT1, adiponectin and leptin behave coherently with FM and there is synchrony between them. The association of SIRT1 with FM is substantially superimposable to that of adiponectin and leptin. Given its homeostatic roles, SIRT1 may deserve to be considered as a plasma clinical/biochemical parameter of adiposity and metabolic health.
Sirtuin 1（SIRT1）是细胞能量可用性的传感器，与瘦素和脂联素一起，它调节代谢稳态。在组织中广泛研究，SIRT1 作为血浆标志物正在评估中。我们旨在评估在缺乏、过量或正常脂肪含量的情况下，循环 SIRT1 是否与瘦素和脂联素一致。评估了 80 名受试者：27 名神经性厌食症（AN），26 名体重正常和 27 名肥胖。评估了血流 SIRT1、瘦素和脂联素（ELISA）、总脂肪量和躯干脂肪量（FM）%、腹部内脏脂肪组织、肝脂肪变性和心外膜脂肪厚度（EFT）。对于每个脂肪储存，使用决定系数（R）评估 SIRT1、瘦素和脂联素的预测能力。血浆 SIRT1 和脂联素与 FM 的增加一致降低，而瘦素则相反。各组之间的每种分析物的平均水平均不同（<0.005）。观察到血浆变量与 FM 之间存在显著相关性。SIRT1 对 FM 具有很好的预测强度，特别是在肥胖组中，EFT 记录了最佳 R（R = 0.7）。血浆 SIRT1、脂联素和瘦素与 FM 一致，它们之间存在同步性。SIRT1 与 FM 的关联与脂联素和瘦素基本相同。鉴于其稳态作用，SIRT1 可能值得被认为是肥胖和代谢健康的血浆临床/生化参数。
