Berková Veronika, Kameniarová Michaela, Ondrisková Vladěna, Berka Miroslav, Menšíková Simona, Kopecká Romana, Luklová Markéta, Novák Jan, Spíchal Lukáš, Rashotte Aaron M, Brzobohatý Břetislav, Černý Martin
Department of Molecular Biology and Radiobiology, Faculty of AgriSciences, Mendel University in Brno, 61300 Brno, Czech Republic.
Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, 77200 Olomouc, Czech Republic.
Plants (Basel). 2020 Nov 13;9(11):1563. doi: 10.3390/plants9111563.
Cytokinins are multifaceted plant hormones that play crucial roles in plant interactions with the environment. Modulations in cytokinin metabolism and signaling have been successfully used for elevating plant tolerance to biotic and abiotic stressors. Here, we analyzed response to INhibitor of CYtokinin DEgradation (INCYDE), a potent inhibitor of cytokinin dehydrogenase. We found that at low nanomolar concentration, the effect of INCYCE on seedling growth and development was not significantly different from that of trans-Zeatin treatment. However, an alteration in the spatial distribution of cytokinin signaling was found at low micromolar concentrations, and proteomics analysis revealed a significant impact on the molecular level. An in-depth proteome analysis of an early (24 h) response and a dose-dependent response after 168 h highlighted the effects on primary and secondary metabolism, including alterations in ribosomal subunits, RNA metabolism, modulations of proteins associated with chromatin, and the flavonoid and phenylpropanoid biosynthetic pathway. The observed attenuation in stress-response mechanisms, including abscisic acid signaling and the metabolism of jasmonates, could explain previously reported positive effects of INCYDE under mild stress conditions.
细胞分裂素是多方面的植物激素，在植物与环境的相互作用中发挥着关键作用。细胞分裂素代谢和信号传导的调节已成功用于提高植物对生物和非生物胁迫的耐受性。在此，我们分析了对细胞分裂素脱氢酶的有效抑制剂细胞分裂素降解抑制剂（INCYDE）的反应。我们发现，在低纳摩尔浓度下，INCYCE对幼苗生长和发育的影响与反式玉米素处理的影响没有显著差异。然而，在低微摩尔浓度下发现细胞分裂素信号的空间分布发生了改变，蛋白质组学分析揭示了在分子水平上的显著影响。对早期（24小时）反应和168小时后剂量依赖性反应的深入蛋白质组分析突出了对初级和次级代谢的影响，包括核糖体亚基的改变、RNA代谢、与染色质相关蛋白质的调节以及类黄酮和苯丙烷生物合成途径。在应激反应机制中观察到的减弱，包括脱落酸信号传导和茉莉酸的代谢，可以解释先前报道的INCYDE在轻度胁迫条件下的积极作用。
