Nejman-Gryz Patrycja, Górska Katarzyna, Paplińska-Goryca Magdalena, Proboszcz Małgorzata, Krenke Rafał
Department of Internal Medicine, Pulmonary Diseases and Allergy, Medical University of Warsaw, Banacha 1a, 02-097 Warsaw, Poland.
J Clin Med. 2020 Nov 15;9(11):3667. doi: 10.3390/jcm9113667.
Periostin and thymic stromal lymphopoietin (TSLP) are newly described markers of obstructive airway diseases and the mechanism by which both markers participate in immune response remains poorly understood. The aim of our study was to determine periostin and TSLP concentration in serum and induced sputum (IS) in patients with atopic asthma, chronic obstructive pulmonary disease (COPD), and controls, as well as to evaluate the potential link between periostin, TSLP, and Th2 immune response. Serum and IS levels of periostin, TSLP, IL-4, and IL-13 were determined in 12 atopic asthmatics, 16 COPD sufferers, and 10 controls. We noticed a significantly higher IS periostin and TSLP concentration at protein and mRNA level in asthmatics compared to the two other groups; additionally, periostin and TSLP were correlated positively with IS eosinophil count. A strong positive correlation between IS periostin and TSLP protein levels (r = 0.96) as well as mRNA expression level (r = 0.95) was found in patients with asthma. The results of our study show that periostin and TSLP are associated with eosinophilic airway inflammation and seem to be important drivers of atopic asthma but not COPD pathobiology. Very strong correlations between local periostin, TSLP, eosinophils, and IL-4 in asthma point to the link between periostin-TSLP and Th2 response.
骨膜蛋白和胸腺基质淋巴细胞生成素（TSLP）是新发现的阻塞性气道疾病标志物，二者参与免疫反应的机制仍不清楚。我们研究的目的是测定特应性哮喘、慢性阻塞性肺疾病（COPD）患者及对照组血清和诱导痰（IS）中骨膜蛋白和TSLP的浓度，并评估骨膜蛋白、TSLP与Th2免疫反应之间的潜在联系。测定了12例特应性哮喘患者、16例COPD患者和10例对照者血清及IS中骨膜蛋白、TSLP、IL-4和IL-13的水平。我们发现，与其他两组相比，哮喘患者IS中骨膜蛋白和TSLP在蛋白质和mRNA水平的浓度显著更高；此外，骨膜蛋白和TSLP与IS嗜酸性粒细胞计数呈正相关。在哮喘患者中，IS骨膜蛋白与TSLP蛋白水平（r = 0.96）以及mRNA表达水平（r = 0.95）之间存在强正相关。我们的研究结果表明，骨膜蛋白和TSLP与嗜酸性粒细胞性气道炎症相关，似乎是特应性哮喘而非COPD病理生物学的重要驱动因素。哮喘中局部骨膜蛋白、TSLP、嗜酸性粒细胞和IL-4之间的极强相关性表明骨膜蛋白 - TSLP与Th2反应之间存在联系。
