Long Noncoding RNA Suppresses Glycolysis and Tumor Progression by Regulating -Mediated Expression in Lung Adenocarcinoma.

BACKGROUND

Lung adenocarcinoma (LUAD) is a leading cause of mortality associated with cancer globally. Thus, it is essential to elucidate its tumorigenesis and prognosis. Accumulating evidence shows that long noncoding RNAs (lncRNAs) play important roles in the occurrence and progression of tumors by regulating their glucose metabolism.

METHODS

Bioinformatics analysis was performed to explore the expression of in LUAD. The level of in LUAD cells and tissues was detected by RT-qPCR. CCK-8, colony formation, EDU and transwell assays were conducted to evaluate the cell growth and migration of LUAD cells (A549 and PC9). High throughput sequencing was used to discover the downstream genes of . The metabolic function of LUAD cells was identified by glucose uptake and lactate production assays. Furthermore, tumor xenografts were established to investigate the effects of on tumor growth in vivo.

RESULTS

Herein, we found that was low-expressed in LUAD, and its level correlated with clinical prognosis. Ectopic expression of inhibited the proliferation and migration of LUAD cells (A549 and PC9). High throughput sequencing and gene enrichment analysis revealed that LINC0551 may be involved in metabolic pathway. Glucose uptake and lactate production assays suggested that suppressed glycolysis of LUAD cells. Mechanistically, our work revealed that inhibited glycolysis in LUAD cells by impairing -mediated transcription of an important glycolysis-related enzyme .

CONCLUSION

In summary, our study identifies as a tumor suppressor in LUAD and implicates the // axis in the glycolytic remodeling of LUAD.