Zhao Lan, Huang Yongdong, Zhu Kai, Miao Zhuang, Zhao Jiazhang, Che Xiang Jing, Hao Dongxia, Zhang Rongyue, Ma Guanghui
State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing P. R. China.
College of Environment and Chemical Engineering Yanshan University Qinhuangdao P. R. China.
Eng Life Sci. 2020 Sep 23;20(11):504-513. doi: 10.1002/elsc.202000023. eCollection 2020 Nov.
Agarose microspheres with a controllable pore structure were manufactured by varying agarose types and crosslinking degrees. Various agarose could tailor the gel formation of microspheres matrix and thus affect the final pore structures. Small pores in microspheres could be fabricated by agarose with a higher molecular weight, which was demonstrated by the packed column with lower distribution coefficient ( ) values measured by gel filtration chromatography. Further, higher values also demonstrated that more and larger pores were formed with increasing the crosslinking degree of agarose microspheres. Either using agarose with a high molecular weight or increasing the crosslinking degree would finally lead to the enhancement of the flow rate during flow performance of packed column as necessary for improving separation efficiency. This provides a foundation for high-resolution chromatography with a controllable separation range as beneficial for downstream process.
通过改变琼脂糖类型和交联度制备了具有可控孔结构的琼脂糖微球。不同的琼脂糖可以调整微球基质的凝胶形成，从而影响最终的孔结构。较高分子量的琼脂糖可制造出微球中的小孔，这通过凝胶过滤色谱法测得的较低分配系数（ ）值的填充柱得以证明。此外，较高的 值还表明，随着琼脂糖微球交联度的增加，会形成更多、更大的孔。使用高分子量的琼脂糖或提高交联度最终都会导致填充柱流动性能期间流速的提高，这对于提高分离效率是必要的。这为具有可控分离范围的高分辨率色谱法奠定了基础，这对下游工艺有益。
