Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus and Faculty of Medicine of Technische Universität Dresden, Germany. Institute of Natural Materials Technology, Faculty of Mechanical Engineering of Technische Universität Dresden, German y.
Biofabrication. 2017 Nov 14;9(4):045011. doi: 10.1088/1758-5090/aa8854.
Plant cell cultures produce active agents for pharmaceuticals, food and cosmetics. However, up to now process control for plant cell suspension cultures is challenging. A positive impact of cell immobilization, such as encapsulation in hydrogel beads, on secondary metabolites production has been reported for several plant species. The aim of this work was to develop a method for bioprinting of plant cells in order to allow fabrication of free-formed three-dimensional matrices with defined internal pore architecture for in depth characterization of immobilization conditions, cell agglomeration and interactions. By using extrusion-based 3D plotting of a basil cell-laden hydrogel blend consisting of alginate, agarose and methylcellulose (alg/aga/mc), we could demonstrate that bioprinting is applicable to plant cells. The majority of the cells survived plotting and crosslinking and the embedded cells showed high viability and metabolic activity during the investigated cultivation period of 20 d. Beside its compatibility with the plant cells, the novel alg/aga/mc blend allowed fabrication of defined 3D constructs with open macropores both in vertical and horizontal direction which were stable under culture conditions for several weeks. Thus, Green Bioprinting, an additive manufacturing technology processing live cells from the plant kingdom, is a promising new immobilization tool for plant cells that enables the development of new bioprocesses for secondary metabolites production as well as monitoring methods.
植物细胞培养生产用于制药、食品和化妆品的活性物质。然而，到目前为止，植物悬浮细胞培养的过程控制仍然具有挑战性。已有报道称，细胞固定化（如包埋在水凝胶珠中）对几种植物物种的次生代谢产物的生产有积极影响。本工作的目的是开发一种用于植物细胞生物打印的方法，以便能够制造具有定义的内部孔结构的自由成型三维基质，从而深入研究固定化条件、细胞团聚和相互作用。通过使用含有海藻酸钠、琼脂糖和甲基纤维素（alg/aga/mc）的罗勒细胞负载水凝胶混合物的基于挤出的 3D 绘图，我们证明了生物打印适用于植物细胞。大多数细胞在绘图和交联过程中存活下来，并且在 20 天的研究培养期间，嵌入的细胞表现出高活力和代谢活性。除了与植物细胞的兼容性外，新型 alg/aga/mc 混合物还允许制造具有开放大孔的定义 3D 结构，这些大孔在垂直和水平方向上均具有稳定性，并且能够在数周的培养条件下保持稳定。因此，Green Bioprinting（一种从植物王国处理活细胞的增材制造技术）是一种有前途的新型植物细胞固定化工具，它可以开发用于次生代谢产物生产以及监测方法的新生物工艺。
