Department of Chemistry & Biology, Dong-Eui University, Busan, Republic of Korea.
Department of Applied Chemistry, Dong-Eui University, Busan, Republic of Korea.
Nat Prod Res. 2022 Feb;36(4):1084-1088. doi: 10.1080/14786419.2020.1849200. Epub 2020 Nov 18.
The aim of this study was to investigate the effect of emodin derived from on melanin production in B16F1 cells. In this study, emodin did not show antioxidant activity in DPPH radical and reducing power assays. However, it was found that emodin scavenged intracellular HO. Emodin increased not only tyrosinase activity but also melanin synthesis in vitro. Moreover, emodin enhanced melanin synthesis by increasing the expression level of tyrosinase (TYR), tyrosine related protein (TRP)-1, TRP-2, MITF and SIRT1 proteins in live cells treated with HO compared with HO treatment group in the analyses of western blot and immunofluorescence. Moreover, emodin suppressed ERK activation by SIRT1 and FOXO1. Thus, emodin promoted melanin synthesis by increasing the expression of TRP-1, TRP-2, tyrosinase through the activation of MITF transcription factor. These findings suggest that emodin could promote melanin production related to anti-hair graying.
本研究旨在探讨大黄素衍生物对 B16F1 细胞黑色素生成的影响。在本研究中，大黄素在 DPPH 自由基和还原力测定中没有显示出抗氧化活性。然而，发现大黄素清除了细胞内的 HO。大黄素不仅增加了酪氨酸酶活性，而且还增加了体外黑色素的合成。此外，与 HO 处理组相比，大黄素在 Western blot 和免疫荧光分析中通过增加 HO 处理细胞中酪氨酸酶 (TYR)、酪氨酸相关蛋白 (TRP)-1、TRP-2、MITF 和 SIRT1 蛋白的表达水平，增强了黑色素的合成。此外，大黄素通过 SIRT1 和 FOXO1 抑制了 ERK 的激活。因此，大黄素通过激活 MITF 转录因子增加 TRP-1、TRP-2、酪氨酸酶的表达来促进黑色素的合成。这些发现表明，大黄素可能通过促进黑色素的生成来预防头发变白。
