不同盖髓材料对脂磷壁酸刺激人牙髓干细胞细胞死亡信号通路的影响。
Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells.
机构信息
Department of Paediatric Dentistry, Faculty of Dentistry, University of Istanbul, Istanbul, Turkey.
Department of Paediatric Dentistry, Faculty of Dentistry, University of Istanbul Kent, Beyoglu, 34433, Istanbul, Turkey.
出版信息
Odontology. 2021 Apr;109(2):547-559. doi: 10.1007/s10266-020-00571-3. Epub 2020 Nov 18.
PURPOSE
This study aimed to evaluate the response of dental pulp stem cells (DPSCs) cultured with and without lipoteichoic acid (LTA) to different pulp-capping materials.
METHODS
The cells were cultured and seeded in 6-well plates and exposed to 1% LTA solution. Dycal, ProRoot MTA and Biodentine materials were applied on cells and all groups were evaluated by cell proliferation, viability, cell cycle and cell death signaling pathways for 24 and 72 h.
RESULTS
LTA + Dycal treatment significantly inhibited the proliferation of DPSCs and increased the apoptosis rate of cells more than the other groups at 72 h. Compared to other groups, LTA + Dycal treatment significantly increased the levels of Caspase-3 and AKT and decreased the levels of p-AKT.
CONCLUSIONS
The results of this study revealed that all tested materials caused apoptosis in DPSCs via an extrinsic apoptotic pathway. The DPSCs showed an early apoptosis response to the Dycal and a late apoptosis response to the ProRoot MTA and Biodentine treatments. LTA led autophagy and inhibited the proliferation of DPSCs. ProRoot MTA and Biodentin eliminated the LTA's bioactivity with higher efficiency than Dycal.
目的
本研究旨在评估培养有和没有脂磷壁酸（LTA）的牙髓干细胞（DPSCs）对不同盖髓材料的反应。
方法
将细胞培养并接种于 6 孔板中，并用 1% LTA 溶液孵育。将 Dycal、ProRoot MTA 和 Biodentine 材料应用于细胞上，并在 24 和 72 小时时通过细胞增殖、活力、细胞周期和细胞死亡信号通路评估所有组。
结果
LTA + Dycal 处理在 72 小时时显著抑制 DPSCs 的增殖，并比其他组增加细胞凋亡率。与其他组相比，LTA + Dycal 处理显著增加了 Caspase-3 和 AKT 的水平，并降低了 p-AKT 的水平。
结论
本研究结果表明，所有测试的材料均通过外在凋亡途径导致 DPSCs 凋亡。DPSCs 对 Dycal 表现出早期凋亡反应，对 ProRoot MTA 和 Biodentine 处理表现出晚期凋亡反应。LTA 导致自噬并抑制 DPSCs 的增殖。ProRoot MTA 和 Biodentine 比 Dycal 更有效地消除 LTA 的生物活性。
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