Machado Vanessa S, Camponogara Camila, Oliveira Sara M, Baldissera Matheus D, Sagrillo Michele R, Gundel Samanta DA S, Silva Ana Paula T DA, Ourique Aline F, Klein Bruna, Wagner Roger, Santos Roberto C V, Silva Aleksandro S DA
Programa de Pós-Graduação em Ciências Farmacêuticas: Análises Clínicas e Toxicológicas, Universidade Federal de Santa Maria/UFSM, Laboratório de Microbiologia Oral, Avenida Roraima, 1000, Prédio 20, Cidade Universitária, Camobi, 97105-900 Santa Maria, RS, Brazil.
Universidade Federal de Santa Maria/UFSM, Departamento de Microbiologia e Parasitologia, Avenida Roraima, 1000, Prédio 20, Cidade Universitária, Camobi, 97105-900 Santa Maria, RS, Brazil.
An Acad Bras Cienc. 2020 Nov 16;92(4):e20191066. doi: 10.1590/0001-3765202020191066. eCollection 2020.
Inflammatory dermatoses are prevalent worldwide, with impacts on the quality of life of patients and their families. The aim of this study was to determine the anti-inflammatory effects of Achyrocline satureioides oily extracts and nanocapsules on the skin using a mouse model of irritant contact dermatitis induced by croton oil, and a skin inflammation model induced by ultraviolet B (UVB) radiation. The mice were treated with 15 mg/ear oily extract (HG-OLAS) or nanocapsules (HG-NCAS) of A. satureioides incorporated into Carbopol® 940 hydrogels. We found that HG-OLAS and HG-NCAS formulations reduced ear edema in croton oil-induced lesions with maximum inhibitions of 54±7% and 74±3%, respectively. HG-OLAS and HG-NCAS formulations decreased ear edema induced by UVB radiation (0.5 J/cm2), with maximum inhibitions of 68±6% and 76±2% compared to the UVB radiation group, respectively. HG-OLAS and HG-NCAS modulated myeloperoxidase (MPO) activity after croton oil induction. Furthermore, croton oil and UVB radiation for 6 and 24 h, respectively, stimulated polymorphonuclear cells infiltration. The topical treatments reduced inflammatory processes, as shown by histological analysis. Together, the data suggest that topical application of A. satureioides oily extracts and nanocapsules produced antiedematogenic and anti-inflammatory effects. They constitute a compelling alternative for treatment of skin injuries.
炎症性皮肤病在全球范围内普遍存在，会对患者及其家人的生活质量产生影响。本研究的目的是使用巴豆油诱导的刺激性接触性皮炎小鼠模型和紫外线B（UVB）辐射诱导的皮肤炎症模型，来确定牛膝草油性提取物和纳米胶囊对皮肤的抗炎作用。将牛膝草的15 mg/耳油性提取物（HG-OLAS）或纳米胶囊（HG-NCAS）掺入卡波姆®940水凝胶中对小鼠进行治疗。我们发现，HG-OLAS和HG-NCAS制剂可减轻巴豆油诱导损伤中的耳部水肿，最大抑制率分别为54±7%和74±3%。HG-OLAS和HG-NCAS制剂可减轻UVB辐射（0.5 J/cm2）诱导的耳部水肿，与UVB辐射组相比，最大抑制率分别为68±6%和76±2%。巴豆油诱导后，HG-OLAS和HG-NCAS可调节髓过氧化物酶（MPO）活性。此外，巴豆油和UVB辐射分别在6小时和24小时刺激了多形核细胞浸润。组织学分析表明，局部治疗可减轻炎症过程。总体而言，数据表明局部应用牛膝草油性提取物和纳米胶囊具有抗水肿和抗炎作用。它们是治疗皮肤损伤的一种有吸引力的替代方法。
