College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, P. R. China.
Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
J Antimicrob Chemother. 2021 Feb 11;76(3):596-600. doi: 10.1093/jac/dkaa483.
To investigate the global distribution, dissemination and overexpression of RE-CmeABC in Campylobacter jejuni.
WGS information for 433 RE-cmeABC-positive C. jejuni isolates (including 18 isolates sequenced in this study and 415 isolates from GenBank) was used for the generation of minimum-spanning trees with STs. WGS information for 95 representative RE-cmeABC-positive C. jejuni isolates was used for phylogenetic analysis. RT-PCR was conducted to evaluate the association between inverted repeat (IR) sequence diversity in the RE-CmeABC promoter region and RE-cmeABC gene expression.
WGS analysis revealed the global distribution of RE-cmeABC among C. jejuni from more than 10 countries. MLST results indicated that various STs were involved in the dissemination of RE-cmeABC, with ST2109 being the most predominant ST. Phylogenetic analysis revealed the close relationship between RE-cmeABC-carrying C. jejuni isolates from poultry and humans. The IR polymorphism in the RE-CmeABC promoter region is associated with the overexpression of RE-cmeABC, which was demonstrated experimentally by RT-PCR.
To the best of our knowledge, our analysis represents the first view of the global distribution of RE-CmeABC, which is horizontally transferable and diffused regionally in a clonal manner. The close relationship of RE-cmeABC-positive C. jejuni from poultry and humans supports the potential of these isolates for zoonotic transmission. Overexpressed RE-CmeABC in C. jejuni will increase the fitness of the corresponding bacteria and be of advantage under antimicrobial selection.
研究空肠弯曲菌中 RE-CmeABC 的全球分布、传播和过表达情况。
利用 433 株 RE-cmeABC 阳性空肠弯曲菌（包括本研究中测序的 18 株和来自 GenBank 的 415 株）的 WGS 信息，生成基于 ST 的最小生成树。利用 95 株具有代表性的 RE-cmeABC 阳性空肠弯曲菌的 WGS 信息进行系统发育分析。采用 RT-PCR 评估 RE-CmeABC 启动子区反向重复（IR）序列多样性与 RE-cmeABC 基因表达之间的关系。
WGS 分析表明，RE-cmeABC 广泛分布于来自 10 多个国家的空肠弯曲菌中。MLST 结果表明，各种 ST 参与了 RE-cmeABC 的传播，其中 ST2109 是最主要的 ST。系统发育分析表明，禽源和人源携带 RE-cmeABC 的空肠弯曲菌密切相关。RE-CmeABC 启动子区的 IR 多态性与 RE-cmeABC 的过表达有关，这通过 RT-PCR 实验得到了证实。
据我们所知，本研究首次分析了 RE-CmeABC 的全球分布情况，该基因可水平转移，并以克隆的方式在区域内扩散。禽源和人源携带 RE-cmeABC 的空肠弯曲菌密切相关，提示这些分离株具有潜在的人畜共患传播能力。空肠弯曲菌中过表达的 RE-CmeABC 将增加相应细菌的适应性，并在抗菌药物选择压力下具有优势。
