Gbx2 鉴定出两种具有不同分子、形态和生理特性的无长突细胞亚型。
Gbx2 Identifies Two Amacrine Cell Subtypes with Distinct Molecular, Morphological, and Physiological Properties.
机构信息
Vollum Institute, Oregon Health and Science University, Portland, OR 97239, USA.
School of Optometry and Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Neuroscience Graduate Program, Oregon Health and Science University, Portland, OR 97239, USA.
出版信息
Cell Rep. 2020 Nov 17;33(7):108382. doi: 10.1016/j.celrep.2020.108382.
Our understanding of nervous system function is limited by our ability to identify and manipulate neuronal subtypes within intact circuits. We show that the Gbx2 mouse line labels two amacrine cell (AC) subtypes in the mouse retina that have distinct morphological, physiological, and molecular properties. Using a combination of RNA-seq, genetic labeling, and patch clamp recordings, we show that one subtype is GABAergic that receives excitatory input from On bipolar cells. The other population is a non-GABAergic, non-glycinergic (nGnG) AC subtype that lacks the expression of standard neurotransmitter markers. Gbx2 nGnG ACs have smaller, asymmetric dendritic arbors that receive excitatory input from both On and Off bipolar cells. Gbx2 nGnG ACs also exhibit spatially restricted tracer coupling to bipolar cells (BCs) through gap junctions. This study identifies a genetic tool for investigating the two distinct AC subtypes, and it provides a model for studying synaptic communication and visual circuit function.
我们对神经系统功能的理解受到我们在完整回路中识别和操纵神经元亚型的能力的限制。我们表明，Gbx2 小鼠品系标记了小鼠视网膜中的两种无长突细胞 (AC) 亚型，它们具有不同的形态、生理和分子特性。我们使用 RNA-seq、遗传标记和膜片钳记录的组合，表明一种亚型是 GABA 能的，它从 On 双极细胞接收兴奋性输入。另一种是非 GABA 能、非甘氨酸能（nGnG）AC 亚型，缺乏标准神经递质标记物的表达。Gbx2 nGnG ACs 具有较小的、不对称的树突树，它从 On 和 Off 双极细胞接收兴奋性输入。Gbx2 nGnG ACs 还通过缝隙连接表现出与双极细胞（BCs）空间限制的示踪剂偶联。这项研究确定了一种用于研究两种不同 AC 亚型的遗传工具，并为研究突触通讯和视觉回路功能提供了模型。
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