Department of Basic Pathology, Sector of Biological Sciences, Federal University of Paraná, PO Box 19031, CEP 81531-990, Curitiba, Paraná, Brazil.
Department of Basic Pathology, Sector of Biological Sciences, Federal University of Paraná, PO Box 19031, CEP 81531-990, Curitiba, Paraná, Brazil.
J Invertebr Pathol. 2020 Nov;177:107503. doi: 10.1016/j.jip.2020.107503. Epub 2020 Nov 15.
The European pepper moth (Duponchelia fovealis) is an invasive pest affecting crops in many countries. The use of chemicals to control D. fovealis is not only ineffective but is hazardous to the environment. The most effective way to reduce this invasive species is biological control using entomopathogenic fungi. Furthermore, the use of combining entomopathogenic fungi is a novel and underexplored approach in the field of biocontrol research. The compatibility of different strains of Beauveria bassiana, Purpureocillium lilacinum, and Isaria javanica was evaluated by forming two-fungi consortia. The pathogenicity of these consortia against D. fovealis, as well as the related enzymatic activities, were investigated. Seven consortia increased D. fovealis mortality, showing synergistic activity. One consortium formed by two strains of B. bassiana produced highest control. Moreover, these consortia also demonstrated increased chitinase and lipase activities. Higher mortality of D. fovealis by these consortia was mainly associated with enzyme production. One consortium, also formed by two strains of B. bassiana, was unique in producing lower D. fovealis mortality than the two strains alone. The potential use of entomopathogenic fungal consortia is a promising alternative approach for biological control. Most of the consortia used in this study improved control of D. fovealis, showed synergistic activity and could be a suitable strategy to control this pest.
欧洲胡椒蛾（Duponchelia fovealis）是一种入侵性害虫，会影响许多国家的农作物。使用化学物质来控制 D. fovealis 不仅无效，而且对环境有害。减少这种入侵物种的最有效方法是使用昆虫病原真菌进行生物防治。此外，使用组合昆虫病原真菌是生物防治研究领域中一种新颖且未充分探索的方法。通过形成两种真菌共生体来评估两种真菌共生体对 Beauveria bassiana、Purpureocillium lilacinum 和 Isaria javanica 的相容性。研究了这些共生体对 D. fovealis 的致病性以及相关酶活性。七种共生体增加了 D. fovealis 的死亡率，表现出协同作用。由两种 B. bassiana 菌株形成的一种共生体产生了最高的控制效果。此外，这些共生体还表现出增加的几丁质酶和脂肪酶活性。这些共生体对 D. fovealis 的更高死亡率主要与酶的产生有关。一种由两种 B. bassiana 菌株形成的共生体也很独特，与单独使用两种菌株相比，它对 D. fovealis 的死亡率较低。昆虫病原真菌共生体的潜在用途是一种有前途的生物防治替代方法。本研究中使用的大多数共生体都提高了对 D. fovealis 的控制效果，表现出协同作用，可能是控制这种害虫的一种合适策略。
