Departamento de Patologia Básica, Setor de Ciências Biológicas, Laboratório de Microbiologia e Biologia Molecular (LabMicro), Universidade Federal do Paraná, Curitiba, Paraná, Brazil.
Departamento de Genética, Laboratório de Genética de Plantas Max Feffer, Escola Superior de Agronomia Luiz de Queiroz - Esalq/USP, Piracicaba, São Paulo, Brazil.
PLoS One. 2022 Jul 14;17(7):e0271460. doi: 10.1371/journal.pone.0271460. eCollection 2022.
The use of two or more microorganisms in a microbial consortium has been increasingly applied in the biological control of diseases and pests. Beauveria bassiana is one of the most widely studied fungal species in biological control, yet little is known about its role in fungal consortiums. In a previous study, our group found that a consortium formed by two strains of B. bassiana had significantly greater biocontrol potential against the polyphagous caterpillars Duponchelia fovealis (Lepidoptera: Crambidae) than either strain on its own. In this study, we use GC-MS and LC-MS/MS to evaluate and discuss the metabolomics of the consortium. A total of 21 consortium biomarkers were identified, corresponding to 14 detected by LC-MS/MS and seven by GC-MS. Antioxidant and anti-inflammatory mechanisms are the main properties of the metabolites produced by the consortium. These metabolites can depress the insect's immune system, increasing its vulnerability and, hence, the fungal virulence of the consortium. In light of these results, we propose an action model of insect mortality due to the metabolites secreted by the consortium. The model includes the inhibition of defense mechanisms such as pro-inflammatory interleukin secretion, cell migration, cell aggregation, Dif, Dorsal and Relish gene transcription, and JAK/STAT and JNK signaling pathways. It also promotes the cleaning of oxidative molecules, like ROS, NOS, and H2O2, and the induction of virulence factors.
在生物防治病虫害方面，越来越多的人开始将两种或两种以上的微生物应用于微生物联合体中。球孢白僵菌是生物防治中研究最广泛的真菌物种之一，但人们对其在真菌联合体中的作用知之甚少。在之前的一项研究中，我们小组发现，由两种球孢白僵菌菌株组成的联合体对多食性毛毛虫（鳞翅目：夜蛾科）的生物防治潜力明显大于单独使用任何一种菌株。在这项研究中，我们使用 GC-MS 和 LC-MS/MS 来评估和讨论联合体的代谢组学。共鉴定出 21 种联合体生物标志物，其中 14 种通过 LC-MS/MS 检测到，7 种通过 GC-MS 检测到。抗氧化和抗炎机制是联合体产生的代谢物的主要特性。这些代谢物可以抑制昆虫的免疫系统，使其更容易受到侵害，从而提高联合体的真菌毒力。鉴于这些结果，我们提出了一个由于联合体分泌的代谢物导致昆虫死亡的作用模型。该模型包括抑制防御机制，如促炎白细胞介素分泌、细胞迁移、细胞聚集、Dif、Dorsal 和 Relish 基因转录以及 JAK/STAT 和 JNK 信号通路。它还促进了氧化分子（如 ROS、NOS 和 H2O2）的清除和毒力因子的诱导。
