CXCR3 及其配体在布氏杆菌性脊柱炎中的作用。

The role of CXCR3 and its ligands expression in Brucellar spondylitis.

机构信息

State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asian, Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang, 830011, P.R. China.

First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830011, P.R. China.

出版信息

BMC Immunol. 2020 Nov 18;21(1):59. doi: 10.1186/s12865-020-00390-9.

DOI:10.1186/s12865-020-00390-9

PMID:33208100

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7672857/

Abstract

AIM

Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS.

METHODS

A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS.

RESULTS

In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls.

CONCLUSION

In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.

摘要

目的

布氏杆菌性脊柱炎（BS）是布氏杆菌病最严重的并发症之一。趋化因子受体 3（CXCR3）与疾病感染的严重程度密切相关。本研究旨在通过分析 BS 患者 CXCR3 及其配体（CXCL9 和 CXCL10）的表达水平，研究 BS 的炎症损伤程度。

方法

共纳入 29 例 BS 患者和 15 名健康对照者。采用实时 PCR 检测 BS 患者和健康对照者外周血单个核细胞（PBMC）中 IFN-γ、CXCR3、CXCL9 和 CXCL10 的 mRNA 表达水平。苏木精-伊红（HE）染色显示 BS 病变组织的病理变化。免疫组织化学染色显示 BS 病变组织中 Brucella-Ab、IFN-γ、CXCR3、CXCL9 和 CXCL10 的蛋白表达水平。同时，采用 ELISA 法检测 BS 患者血清中 IFN-γ、CXCL9、CXCL10 和 CXCR3 自身抗体的水平。

结果

BS 患者病变组织中出现软骨坏死、急性或慢性炎症浸润。Brucella-Ab 蛋白在密切相关的病变组织中大量表达。同时，BS 患者密切相关的病变组织和血清中 IFN-γ、CXCR3 和 CXCL10 的蛋白表达水平均较高。同时，BS 患者 PBMC 中 IFN-γ、CXCR3 和 CXCL10 的 mRNA 表达水平明显高于对照组。