Shang Xiaoqian, Wang Liang, Liu Yumei, Liu Xuemei, Lv Jie, Zhou Xuan, Wang Hao, Nazierhan Shaxika, Wang Jing, Ma Xiumin
Clinical Laboratory Center, Tumor Hospital Affiliated to Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
State Key Laboratory of Pathogenesis, Prevention and Treatment of High Incidence Diseases in Central Asia, First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830011, P.R. China.
Exp Ther Med. 2021 Jan;21(1):73. doi: 10.3892/etm.2020.9505. Epub 2020 Nov 25.
The present study aimed to investigate whether C-X-C motif chemokine receptor 3 (CXCR3) and its ligands may aid in diagnosing spinal tuberculosis (ST). A total of 36 patients with ST and 20 healthy controls were enrolled in the present study. The morphology of tuberculous granuloma in spinal tissue was observed by hematoxylin and eosin staining. The presence and distribution of acid-fast bacilli (AFB) were observed by Ziehl-Neelsen (ZN) staining. The protein expression of Ag85B, IFN-γ, and CXCR3 and its ligands (CXCL9 and CXCL10) were detected by immunohistochemistry. The levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST and healthy controls were detected by reverse transcription-quantitative polymerase chain reaction and ELISA. Typical tuberculous granuloma was observed in the ST close tissue. AFB was observed by ZN staining. Positive expression of Ag85B was found in the surrounding caseous necrotic tissue of the tuberculous granuloma. IFN-γ, CXCR3, CXCL9 and CXCL10 were expressed in the tissue surrounding the tuberculous granuloma and their expression levels were markedly higher than those in the distant tissues. The levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood of patients with ST were significantly higher than those in the healthy controls. Receiver operating characteristic curve analysis demonstrated that IFN-γ, CXCR3 and CXCL10 were more reliable diagnostic markers in terms of sensitivity and specificity. IFN-γ, CXCR3, CXCL9 and CXCL10 were highly expressed in the lesion tissue and peripheral blood samples of patients with ST, and IFN-γ, CXCR3 and its ligands aided in diagnosing ST.
本研究旨在探讨C-X-C基序趋化因子受体3（CXCR3）及其配体是否有助于脊柱结核（ST）的诊断。本研究共纳入36例ST患者和20名健康对照者。通过苏木精-伊红染色观察脊柱组织中结核性肉芽肿的形态。通过萋-尼（ZN）染色观察抗酸杆菌（AFB）的存在及分布。采用免疫组织化学法检测Ag85B、IFN-γ、CXCR3及其配体（CXCL9和CXCL10）的蛋白表达。采用逆转录-定量聚合酶链反应和酶联免疫吸附测定法检测ST患者和健康对照者外周血中IFN-γ、CXCR3、CXCL9和CXCL10的水平。在ST临近组织中观察到典型的结核性肉芽肿。通过ZN染色观察到AFB。在结核性肉芽肿周围的干酪样坏死组织中发现Ag85B阳性表达。IFN-γ、CXCR3、CXCL9和CXCL10在结核性肉芽肿周围组织中表达，其表达水平明显高于远处组织。ST患者外周血中IFN-γ、CXCR3、CXCL9和CXCL10的水平明显高于健康对照者。受试者工作特征曲线分析表明，IFN-γ、CXCR3和CXCL10在敏感性和特异性方面是更可靠的诊断标志物。IFN-γ、CXCR3、CXCL9和CXCL10在ST患者的病变组织和外周血样本中高表达，且IFN-γ、CXCR3及其配体有助于ST的诊断。
