Wangsa Khamhee, Sarma Indira, Saikia Purbajyoti, Ananthakrishnan Dhanabalan, Sarma Hirendra Nath, Velmurugan Devadasan
Department of Zoology, Rajiv Gandhi University, Rono Hills, Itanagar, Arunachal Pradesh, India.
Centre of Advanced Study in Crystallography and Biophysics, University of Madras, Guindy Campus, Chennai, India.
J Reprod Infertil. 2020 Oct-Dec;21(4):247-258. doi: 10.18502/jri.v21i4.4329.
Linn. is reported to be used by women of Assam and Arunachal Pradesh in northeast India for treating menstrual disorders. contains compounds that bind with estrogen receptors (ERα and ERβ) evidenced by increased PCNA in endometrial epithelium.
Crude extract was orally administered at the dose of 500 body weight/day to the female mice (60-70 days old) in five different groups. Each group containing six females included: (I) cyclic control, (II) cyclic extract treated, (III) Ovariectomized (OVX)-vehicle treated (Control), (IV) OVX-E2 treated (V) OVX- extract treated. Extract was administered for eight days to the cyclic groups and three days to the OVX groups. PCNA was detected immunohistochemically in uterine tissues and signals were analyzed by Image J software (NIH, USA). Compounds were separated by GC-MS and identified using NIST. In silico molecular docking studies was performed with human estrogen receptors (ERα and ERβ). Molecular dynamics (MD) simulations of the best interacting compound was done using gromacs.
The results showed cell proliferation in the uterine endometrium evidenced by PCNA. Two phytocompounds, Octadecanoic acid and methyl stearate showed binding affinity with ERα and ERβ.
contains compounds having binding affinity with ERα and ERβ. The present study is the first report on compounds from showing binding affinity with human estrogen receptors which may have biological effect on female reproduction.
据报道，印度东北部阿萨姆邦和阿鲁纳恰尔邦的女性使用[植物名称未给出]来治疗月经紊乱。[植物名称未给出]含有的化合物可与雌激素受体（ERα和ERβ）结合，子宫内膜上皮细胞中增殖细胞核抗原（PCNA）增加证明了这一点。
将粗提物以500毫克/千克体重/天的剂量口服给予五个不同组的雌性小鼠（60 - 70日龄）。每组包含六只雌性小鼠，包括：（I）周期对照，（II）周期提取物处理组，（III）去卵巢（OVX） - 载体处理组（对照），（IV）OVX - 雌二醇（E2）处理组，（V）OVX - 提取物处理组。提取物给予周期组八天，给予OVX组三天。通过免疫组织化学法在子宫组织中检测PCNA，并使用Image J软件（美国国立卫生研究院）分析信号。化合物通过气相色谱 - 质谱联用（GC - MS）分离，并使用美国国家标准与技术研究院（NIST）进行鉴定。对人雌激素受体（ERα和ERβ）进行了计算机模拟分子对接研究。使用格罗麦斯（gromacs）软件对最佳相互作用化合物进行分子动力学（MD）模拟。
结果显示，PCNA证明子宫内膜有细胞增殖。两种植物化合物，十八烷酸和硬脂酸甲酯显示出与ERα和ERβ的结合亲和力。
[植物名称未给出]含有与ERα和ERβ具有结合亲和力的化合物。本研究是关于[植物名称未给出]中化合物与人类雌激素受体具有结合亲和力的首次报道，这些化合物可能对女性生殖有生物学作用。
