Cornacchia Alessandra, Saletti Maria Antonietta, Di Marzio Violeta, Salini Romolo, Marfoglia Cristina, Tieri Elga, D'Alterio Nicola, Marri Nicla, Losito Francesca, Arienzo Alyexandra, Murgia Lorenza, Antonini Giovanni, Amatiste Simonetta, Leboffe Loris, Pomilio Francesco
Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo.
Istituto Zooprofilattico Sperimentale del Lazio e della Toscana M. Aleandri, Rome.
Ital J Food Saf. 2020 Nov 4;9(3):7696. doi: 10.4081/ijfs.2020.7696. eCollection 2020 Oct 16.
The aim of this study was to evaluate the performance of "Micro Biological Survey - MBS Test" in the enumeration of bacterial load in cow raw milk. The MBS test is based on a colorimetric method recently developed and patented by "Roma Tre" University, Italy. The evaluation of the performance of the MBS method was carried out by comparison with plate count at 30°C (gold standard) and flow cytometry. Thirteen independent set of experiments were performed analyzing a total of 104 samples of cow raw milk with the selected methods. Results obtained using the MBS method are comparable with those obtained with the plate count method at 30°C (CFU/mL) and flow cytometry technology; in particular, the results obtained with the MBS method are very close to plate count's at 30°C. On the other hand, there are statistically significant differences between these two methods' and flow cytometry technology's results that could be due to the different experimental conditions.
本研究的目的是评估“微生物检测 - MBS检测”在测定生鲜牛奶中细菌载量方面的性能。MBS检测基于意大利“罗马第三大学”最近开发并获得专利的比色法。通过与30°C平板计数法（金标准）和流式细胞术进行比较，对MBS方法的性能进行了评估。使用选定的方法对13组独立实验进行了分析，共分析了104份生鲜牛奶样本。使用MBS方法获得的结果与30°C平板计数法（CFU/mL）和流式细胞术技术获得的结果具有可比性；特别是，使用MBS方法获得的结果与30°C平板计数法的结果非常接近。另一方面，这两种方法与流式细胞术技术的结果之间存在统计学上的显著差异，这可能是由于实验条件不同所致。
