Rio M C, Bellocq J P, Gairard B, Rasmussen U B, Krust A, Koehl C, Calderoli H, Schiff V, Renaud R, Chambon P
Laboratoire de Génétique Moléculaire, Centre National de la Recherche Scientifique et U. 184, Faculté de Médecine, Strasbourg, France.
Proc Natl Acad Sci U S A. 1987 Dec;84(24):9243-7. doi: 10.1073/pnas.84.24.9243.
The expression of the pS2 gene, which is induced by estrogen in the breast cancer cell line MCF-7, has been investigated in breast cancers by using pS2 mRNA determination in tumor specimens and immunocytochemistry to identify pS2 protein in paraffin-embedded sections. Using these assays we show that determination of pS2 gene expression allows the definition of subclasses of estrogen-receptor-containing breast cancers that may be used to more precisely identify estrogen-dependent tumors. Tumor specimens have also been analyzed for the presence of mRNAs for the estrogen receptor and for the ERBB2 oncogene. No evidence for the presence of truncated forms of estrogen-receptor mRNA has been found, and overexpression of the ERBB2 oncogene did not correlate with the steroid receptor status or pS2 gene expression.
通过在肿瘤标本中检测pS2 mRNA以及利用免疫细胞化学方法在石蜡包埋切片中鉴定pS2蛋白，对雌激素诱导乳腺癌细胞系MCF-7中pS2基因的表达情况进行了研究。通过这些检测，我们发现，检测pS2基因表达有助于定义含雌激素受体的乳腺癌亚类，从而更精确地识别雌激素依赖性肿瘤。还对肿瘤标本进行了雌激素受体和ERBB2癌基因mRNA的检测。未发现雌激素受体mRNA截短形式存在的证据，且ERBB2癌基因的过表达与类固醇受体状态或pS2基因表达无关。
