Masiakowski P, Breathnach R, Bloch J, Gannon F, Krust A, Chambon P
Nucleic Acids Res. 1982 Dec 20;10(24):7895-903. doi: 10.1093/nar/10.24.7895.
cDNA clones corresponding to a mRNA whose level is rapidly increased by addition of oestradiol to the culture medium have been isolated by differential screening of a cDNA library from the breast cancer cell line MCF-7, which contains oestrogen receptors. Such clones will be useful in studies of the DNA sequences required for hormonal induction and to determine whether expression of the corresponding gene is in any way related to the cancerous state. We have also obtained a cDNA clone for a messenger whose level is apparently decreased by steroid hormones.
通过对含有雌激素受体的乳腺癌细胞系MCF-7的cDNA文库进行差异筛选，已分离出与一种mRNA相对应的cDNA克隆，该mRNA的水平在向培养基中添加雌二醇后会迅速升高。此类克隆将有助于研究激素诱导所需的DNA序列，并确定相应基因的表达是否在任何方面与癌状态相关。我们还获得了一种信使RNA的cDNA克隆，其水平明显受到类固醇激素的降低。
