c-erbB2、转化生长因子-β1和pS2基因在原发性人类乳腺癌中的表达

Expression of c-erbB2, TGF-beta 1 and pS2 genes in primary human breast cancers.

作者信息

Delvenne C G, Winkler-Gol R A, Piccart M J, Hustin J, Michaux D, Leclercq G, Nogaret J M, Autier P

机构信息

Laboratory of Molecular Oncology, University of Liège-Sart Tilman, Belgium.

出版信息

Eur J Cancer. 1992;28(2-3):700-5. doi: 10.1016/s0959-8049(05)80130-3.

DOI:10.1016/s0959-8049(05)80130-3

PMID:1350458

Abstract

The presence of c-erbB2, TGF-beta 1 and pS2 mRNAs was examined in primary breast tumours. The c-erbB2 mRNA was overexpressed in 34% of the tumours. There was a positive, statistically significant correlation between c-erbB2 gene overexpression and nodal status. TGF-beta 1 mRNA was detected in 84% of the tumours, regardless of their clinical status. When possible, the c-erbB2 and TGF-beta 1 proteins were identified immunohistochemically on frozen sections from the same tumours. For TGF-beta 1, the mRNA and immunohistochemical results were divergent in 6 cases, 5 of which did contain clearly detectable mRNA but did not stain with the antibody. The pS2 mRNA was detected in 22% of the tumours and in the BT474 cell line. There was a significant correlation between the presence of pS2 mRNA and of oestrogen receptors. No statistically significant correlation was observed between pS2 and TGF-beta 1 genes expression and the clinical parameters of the tumours.

摘要

在原发性乳腺肿瘤中检测了c-erbB2、转化生长因子-β1（TGF-β1）和pS2 mRNA的存在情况。34%的肿瘤中c-erbB2 mRNA过表达。c-erbB2基因过表达与淋巴结状态之间存在正相关，且具有统计学意义。84%的肿瘤中检测到TGF-β1 mRNA，无论其临床状态如何。在可能的情况下，对同一肿瘤的冰冻切片进行免疫组织化学鉴定c-erbB2和TGF-β1蛋白。对于TGF-β1，mRNA和免疫组织化学结果在6例中存在差异，其中5例确实含有明显可检测到的mRNA，但未被抗体染色。22%的肿瘤以及BT474细胞系中检测到pS2 mRNA。pS2 mRNA的存在与雌激素受体之间存在显著相关性。未观察到pS2和TGF-β1基因表达与肿瘤临床参数之间存在统计学意义的相关性。