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分析超速离心法:仍是提供多种解决方案的金标准。

Analytical ultracentrifugation: still the gold standard that offers multiple solutions.

机构信息

Biomolecular Interaction Centre and School of Biological Sciences, University of Canterbury, PO Box 4800, Christchurch, 8140, New Zealand.

Bio21 Molecular Science and Biotechnology Institute, Department of Biochemistry and Molecular Biology, University of Melbourne, Parkville, VIC, 3010, Australia.

出版信息

Eur Biophys J. 2020 Dec;49(8):673-676. doi: 10.1007/s00249-020-01483-4.

Abstract

Understanding the nature of macromolecules and their interactions in solution underpins many fields, including biology, chemistry and materials science. The 24th International Analytical Ultracentrifugation Workshop and Symposium (AUC2019, held in Christchurch, New Zealand, August 2019), brought together 77 international delegates to highlight recent developments in the field. There was a focus on analytical ultracentrifugation, although we recognise that this is but one of the key methods in the biophysicist's toolkit. Many of the presentations showcased the versatility of analytical ultracentrifugation and how such experiments are integrated with other solution techniques, such as small-angle X-ray scattering, cryo-electron microscopy, isothermal titration calorimetry and more. This special issue emphasises a wide range of themes covered in the meeting, including carbohydrate chemistry, protein chemistry, polymer science, and macromolecular interactions.

摘要

理解大分子的性质及其在溶液中的相互作用是许多领域的基础,包括生物学、化学和材料科学。第 24 届国际分析超速离心研讨会和专题讨论会(AUC2019,2019 年 8 月在新西兰克赖斯特彻奇举行)汇集了 77 名国际代表,重点介绍了该领域的最新进展。会议的重点是分析超速离心,但我们认识到这只是生物物理学家工具包中的关键方法之一。许多演讲展示了分析超速离心的多功能性,以及如何将此类实验与其他溶液技术(如小角度 X 射线散射、低温电子显微镜、等温滴定量热法等)相结合。本期特刊强调了会议涵盖的广泛主题,包括碳水化合物化学、蛋白质化学、聚合物科学和大分子相互作用。

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