分析超速离心机：非编码 RNA 特性分析的理想工具。
Analytical ultracentrifuge: an ideal tool for characterization of non-coding RNAs.
机构信息
Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 4401 University Dr. West, Lethbridge, AB, T1K 3M4, Canada.
Department of Microbiology, Immunology and Infectious Diseases, Cumming, School of Medicine, University of Calgary, 2500 University Dr. North West, Calgary, AB, T2N 1N4, Canada.
出版信息
Eur Biophys J. 2020 Dec;49(8):809-818. doi: 10.1007/s00249-020-01470-9. Epub 2020 Oct 16.
Analytical ultracentrifugation (AUC) has emerged as a robust and reliable technique for biomolecular characterization with extraordinary sensitivity. AUC is widely used to study purity, conformational changes, biomolecular interactions, and stoichiometry. Furthermore, AUC is used to determine the molecular weight of biomolecules such as proteins, carbohydrates, and DNA and RNA. Due to the multifaceted role(s) of non-coding RNAs from viruses, prokaryotes, and eukaryotes, research aimed at understanding the structure-function relationships of non-coding RNAs is rapidly increasing. However, due to their large size, flexibility, complicated secondary structures, and conformations, structural studies of non-coding RNAs are challenging. In this review, we are summarizing the application of AUC to evaluate the homogeneity, interactions, and conformational changes of non-coding RNAs from adenovirus as well as from Murray Valley, Powassan, and West Nile viruses. We also discuss the application of AUC to characterize eukaryotic long non-coding RNAs, Xist, and HOTAIR. These examples highlight the significant role AUC can play in facilitating the structural determination of non-coding RNAs and their complexes.
分析超速离心（AUC）已成为一种强大而可靠的技术，可用于生物分子的特征分析，具有极高的灵敏度。AUC 广泛用于研究纯度、构象变化、生物分子相互作用和化学计量。此外，AUC 还用于测定生物分子（如蛋白质、碳水化合物、DNA 和 RNA）的分子量。由于病毒、原核生物和真核生物的非编码 RNA 具有多方面的作用，因此旨在研究非编码 RNA 的结构-功能关系的研究正在迅速增加。然而，由于非编码 RNA 体积大、结构灵活、二级结构和构象复杂，因此对其进行结构研究具有挑战性。在这篇综述中，我们总结了 AUC 在评估腺病毒以及默里谷病毒、波瓦桑病毒和西尼罗河病毒中非编码 RNA 的均一性、相互作用和构象变化方面的应用。我们还讨论了 AUC 在表征真核长非编码 RNA Xist 和 HOTAIR 方面的应用。这些例子突出了 AUC 在促进非编码 RNA 及其复合物的结构测定方面的重要作用。
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