Disruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, 1 Create Way, #03-06/07/8 Research Wing, Singapore, 138602, Singapore.
Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Singapore.
Sci Rep. 2020 Nov 19;10(1):20206. doi: 10.1038/s41598-020-76485-5.
Precision agriculture requires new technologies for rapid diagnosis of plant stresses, such as nutrient deficiency and drought, before the onset of visible symptoms and subsequent yield loss. Here, we demonstrate a portable Raman probe that clips around a leaf for rapid, in vivo spectral analysis of plant metabolites including carotenoids and nitrates. We use the leaf-clip Raman sensor for early diagnosis of nitrogen deficiency of the model plant Arabidopsis thaliana as well as two important vegetable crops, Pak Choi (Brassica rapa chinensis) and Choy Sum (Brassica rapa var. parachinensis). In vivo measurements using the portable leaf-clip Raman sensor under full-light growth conditions were consistent with those obtained with a benchtop Raman spectrometer measurements on leaf-sections under laboratory conditions. The portable leaf-clip Raman sensor offers farmers and plant scientists a new precision agriculture tool for early diagnosis and real-time monitoring of plant stresses in field conditions.
精准农业需要新技术来快速诊断植物胁迫，例如在出现可见症状和随后的产量损失之前诊断营养缺乏和干旱。在这里，我们展示了一种便携式拉曼探头，它可以夹在叶子上，快速进行植物代谢物的体内光谱分析，包括类胡萝卜素和硝酸盐。我们使用叶片夹式拉曼传感器来早期诊断模式植物拟南芥以及两种重要蔬菜作物白菜（ Brassica rapa chinensis ）和蕹菜（ Brassica rapa var. parachinensis ）的氮缺乏症。在全光照生长条件下使用便携式叶片夹式拉曼传感器进行的体内测量与在实验室条件下用台式拉曼光谱仪对叶片切片进行的测量结果一致。便携式叶片夹式拉曼传感器为农民和植物科学家提供了一种新的精准农业工具，可用于在田间条件下对植物胁迫进行早期诊断和实时监测。
