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INTRODUCTION

Wound dressings often fall short of providing the multifunctional capabilities required for optimal wound healing, such as promoting cell migration, proliferation, and tissue regeneration. Decellularization of plant tissues has gained attention as a potential source of biomaterials for tissue engineering applications due to their favorable characteristics, including pre-existing vascular networks, interconnected porous structure, efficient water transport and retention, high surface area, and a diverse range of mechanical properties.

METHODS

This study investigates the feasibility of using decellularized walnut leaves (DWL) as a novel scaffold for wound dressing in a mice model of excisional wounds. The decellularization and bleaching processes were carried out using various chemical agents. DNA and protein quantification and hematoxylin and eosin staining were performed to reveal the successful removal of cells in DWL. Scanning electron microscopy (SEM) was used to indicate that the normal structure of walnut leaves was preserved after chemical decellularization. Chemical characterization was conducted using Fourier-transform infrared (FTIR) and Raman spectroscopy to show the remaining bioactive molecules and components in the structure of DWL.

RESULTS

Comparing tensile strength and surface roughness parameters, surface wettability, swelling, and porosity properties of native and DWL indicated no statistical differences between them. SEM analysis demonstrated that human mesenchymal stem cells excellently attach and proliferate on the DWL. Additionally, the biocompatibility and potential of DWL scaffolds to accelerate wound closure and enhance histopathological scores, collagen deposition, and epithelial thickness were observed in a mice model of excisional wounds.

DISCUSSION

In conclusion, DWL shows promising potential for application as a skin wound dressing due to its biocompatibility, ability to promote cell attachment and proliferation, and efficacy in accelerating wound healing.

INTRODUCTION

METHODS

RESULTS

DISCUSSION

引言

伤口敷料往往无法提供最佳伤口愈合所需的多功能能力，如促进细胞迁移、增殖和组织再生。植物组织脱细胞化作为组织工程应用生物材料的潜在来源已受到关注，因为它们具有有利的特性，包括预先存在的血管网络、相互连接的多孔结构、高效的水运输和保留、高表面积以及多样的机械性能。

方法

本研究在切除伤口的小鼠模型中研究了使用脱细胞化核桃叶（DWL）作为新型伤口敷料支架的可行性。使用各种化学试剂进行脱细胞化和漂白过程。进行DNA和蛋白质定量以及苏木精和伊红染色以揭示DWL中细胞的成功去除。扫描电子显微镜（SEM）用于表明化学脱细胞化后核桃叶的正常结构得以保留。使用傅里叶变换红外（FTIR）和拉曼光谱进行化学表征，以显示DWL结构中剩余的生物活性分子和成分。