Department of Oncology, The First Affiliated Hospital of Gannan Medical University, Ganzhou, China.
Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):11114-11119. doi: 10.26355/eurrev_202011_23598.
To determine expressions of MicroRNA-19a-3p (miRNA-19a-3p) and PDCD5 in nasopharyngeal carcinoma (NPC) tissues, and their prognostic potentials in NPC.
Expressions of miRNA-19a-3p and PDCD5 in NPC tissues and controls were determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between expressions of miRNA-19a-3p and PDCD5 in NPC was evaluated by Pearson correlation test. Furthermore, potential influences of miRNA-19a-3p and PDCD5 on clinical features of NPC patients were assessed. Through 5-year follow-up, survival analysis in NPC patients was conducted by Kaplan-Meier method. Finally, factors influencing prognosis of NPC were determined using the Cox regression model.
MiRNA-19a-3p was upregulated and PDCD5 was downregulated in NPC tissues. Pearson correlation test uncovered a negative correlation between expression levels of miRNA-19a-3p and PDCD5 in NPC tissues. MiRNA-19a-3p level was correlated with N classification and clinical stage in NPC patients, while PDCD5 level was correlated with T classification, pathological grade and clinical stage. Survival analysis showed poor prognosis in NPC patients expressing high level of miRNA-19a-3p or low level of PDCD5. Cox regression analysis illustrated that N2+3 classification, clinical stage III+IV, high level of miRNA-19a-3p and low level of PDCD5 were independent risk factors for the prognosis of NPC.
MiRNA-19a-3p is upregulated and PDCD5 is downregulated in NPC tissues. High level of miRNA-19a-3p and low level of PDCD5 are unfavorable for the prognosis of NPC.
检测微小 RNA-19a-3p（miRNA-19a-3p）和 PDCD5 在鼻咽癌（NPC）组织中的表达及其在 NPC 中的预后潜力。
采用实时定量聚合酶链反应（qRT-PCR）检测 NPC 组织和对照组织中 miRNA-19a-3p 和 PDCD5 的表达。采用 Pearson 相关检验评估 NPC 中 miRNA-19a-3p 和 PDCD5 的表达相关性。进一步评估 miRNA-19a-3p 和 PDCD5 对 NPC 患者临床特征的潜在影响。通过 5 年随访，采用 Kaplan-Meier 法对 NPC 患者进行生存分析。最后，采用 Cox 回归模型确定影响 NPC 预后的因素。
miRNA-19a-3p 在 NPC 组织中上调，PDCD5 下调。Pearson 相关检验揭示 NPC 组织中 miRNA-19a-3p 和 PDCD5 的表达水平呈负相关。miRNA-19a-3p 水平与 NPC 患者的 N 分类和临床分期相关，而 PDCD5 水平与 T 分类、病理分级和临床分期相关。生存分析显示，表达高水平 miRNA-19a-3p 或低水平 PDCD5 的 NPC 患者预后不良。Cox 回归分析表明，N2+3 分类、临床分期 III+IV、高水平 miRNA-19a-3p 和低水平 PDCD5 是 NPC 预后的独立危险因素。
miRNA-19a-3p 在 NPC 组织中上调，PDCD5 下调。高水平的 miRNA-19a-3p 和低水平的 PDCD5 不利于 NPC 的预后。
