Department of ENT and HN Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, China.
Eur Rev Med Pharmacol Sci. 2020 Aug;24(16):8402-8407. doi: 10.26355/eurrev_202008_22637.
The purpose of this study was to uncover the correlations of expression levels of microRNA-429 (miRNA-429) and SOX2 with clinical parameters and prognosis of nasopharyngeal carcinoma.
Nasopharyngeal carcinoma patients (n=95) and nasopharyngitis patients (n=95) in the same period were enrolled. The relative levels of miRNA-429 and SOX2 in nasopharyngeal tissues collected from these patients were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Then, the potential correlation between miRNA-429 and SOX2 was analyzed by Pearson correlation test. Next, the influences of miRNA-429 and SOX2 levels on clinical parameters of nasopharyngeal carcinoma patients were assessed. At last, the factors influencing the prognosis of nasopharyngeal carcinoma were determined using the Cox regression model.
It was found that downregulated miRNA-429 and upregulated SOX2 were observed in nasopharyngeal tissues collected from nasopharyngeal carcinoma patients. MiRNA-429 level was negatively correlated with that of SOX2 in nasopharyngeal carcinoma tissues. In addition, miRNA-429 and SOX2 levels were related to age, tumor differentiation, T stage, N stage, and clinical grade of nasopharyngeal carcinoma patients. Moreover, worse prognosis was seen in nasopharyngeal carcinoma patients expressing low level of miRNA-429 or high level of SOX2. Furthermore, Cox regression analysis showed that T3-T4 stage, moderate to high differentiation, and high level of SOX2 were risk factors, while high level of miRNA-429 was the protective factor for nasopharyngeal carcinoma.
Downregulation of miRNA-429 and upregulation of SOX2 are unfavorable to the prognosis of nasopharyngeal carcinoma.
本研究旨在揭示 microRNA-429（miRNA-429）和 SOX2 的表达水平与鼻咽癌临床参数和预后的相关性。
纳入同期鼻咽癌患者（n=95）和鼻咽部炎症患者（n=95），采用逆转录-聚合酶链反应（RT-PCR）检测收集的鼻咽组织中 miRNA-429 和 SOX2 的相对水平，采用 Pearson 相关检验分析 miRNA-429 和 SOX2 之间的潜在相关性，评估 miRNA-429 和 SOX2 水平对鼻咽癌患者临床参数的影响，采用 Cox 回归模型确定影响鼻咽癌预后的因素。
发现鼻咽癌患者鼻咽组织中存在下调的 miRNA-429 和上调的 SOX2。miRNA-429 水平与鼻咽癌组织中 SOX2 水平呈负相关。此外，miRNA-429 和 SOX2 水平与鼻咽癌患者的年龄、肿瘤分化程度、T 分期、N 分期和临床分级有关。而且，miRNA-429 水平低或 SOX2 水平高的鼻咽癌患者预后较差。进一步的 Cox 回归分析显示，T3-T4 期、中高度分化和 SOX2 水平高是危险因素，而 miRNA-429 水平高是鼻咽癌的保护因素。
miRNA-429 下调和 SOX2 上调不利于鼻咽癌的预后。
