依托咪酯抑制耐甲氧西林金黄色葡萄球菌的生长，并与苯唑西林表现出协同作用。

Etomidate inhibits the growth of MRSA and exhibits synergism with oxacillin.

机构信息

Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.

Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.

出版信息

Future Microbiol. 2020 Nov;15:1611-1619. doi: 10.2217/fmb-2020-0078. Epub 2020 Nov 20.

DOI:10.2217/fmb-2020-0078

PMID:33215536

Abstract

The purpose of this study was to evaluate the antimicrobial activity of the anesthetic etomidate against strains of MRSA and biofilms. The antibacterial effect of etomidate was assessed by the broth microdilution method. To investigate the probable action mechanism of the compound flow cytometry techniques were used. MRSA strains showed MIC equal to 500 and 1000 μg/ml of etomidate. Four-fifths (80%) of the tested MRSA strains demonstrated synergistic effect with oxacillin. Etomidate also showed activity against MRSA biofilm at concentration of 250 μg/ml. Cytometric analysis revealed that the cells treated with etomidate leading to cell death, probably by apoptosis. Etomidate showed antibacterial activity against MRSA.

摘要

本研究旨在评估麻醉药依托咪酯对耐甲氧西林金黄色葡萄球菌（MRSA）菌株和生物膜的抗菌活性。通过肉汤微量稀释法评估依托咪酯的抗菌作用。为了研究该化合物的可能作用机制，采用了流式细胞术技术。MRSA 菌株对依托咪酯的 MIC 值等于 500 和 1000μg/ml。检测到的 MRSA 菌株中有五分之四（80%）与苯唑西林表现出协同作用。依托咪酯在 250μg/ml 的浓度下也对 MRSA 生物膜表现出活性。细胞计量分析显示，用依托咪酯处理的细胞导致细胞死亡，可能是通过细胞凋亡。依托咪酯对 MRSA 具有抗菌活性。