Department of Clinical & Toxicological Analysis, School of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza, CE, Brazil.
Drug Research & Development Center, Federal University of Ceará, Fortaleza, CE, Brazil.
Future Microbiol. 2021 Apr;16:375-387. doi: 10.2217/fmb-2020-0095. Epub 2021 Apr 19.
To evaluate the activity of diclofenac sodium and synergism with oxacillin against clinical strains of SARM in plactonic cells, antibiofilm and biofilm. Synergism activity was assessed using the fractional inhibitory concentration index and its possible mechanism of action by flow cytometry. The synergistic activity of diclofenac sodium with oxacillin was observed against plactonic cells, antibiofilm and in biofilm formed from clinical methicillin-resistant strains. This combination caused damage to the integrity of the membrane and ruptures in the DNA of the cells, leading to apoptosis.
为了评估双氯芬酸钠与苯唑西林联合应用对浮游细胞、生物膜和生物膜中临床分离的耐甲氧西林金黄色葡萄球菌（SARM）菌株的活性，采用微量稀释法测定了氟氯西林与双氯芬酸钠的协同抑菌作用，并用流式细胞术分析了其作用机制。双氯芬酸钠与苯唑西林联合应用对浮游细胞、抗生物膜和临床分离的耐甲氧西林金黄色葡萄球菌生物膜均有协同作用。该联合用药可破坏细胞膜的完整性，导致细胞内 DNA 断裂，引起细胞凋亡。
