State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang 550002, China.
Guangzhou Huali Science and Technology Vocational College, Guangzhou 511325, China.
J Control Release. 2020 Jun 10;322:187-199. doi: 10.1016/j.jconrel.2020.03.010. Epub 2020 Mar 10.
Paper is a common material that is promising for constructing microfluidic chips (lab-on-a-paper) for diagnostics and drug delivery for biomedical applications. In the past decade, extensive research on paper-based microfluidics has accumulated a large number of scientific publications in the fields of biomedical diagnosis, food safety, environmental health, drug screening and delivery. This review focuses on the recent progress on paper-based microfluidic technology with an emphasis on the design, optimization and application of the technology platform, in particular for medical diagnostics and drug delivery. Novel advances have concentrated on engineering paper devices for point-of-care (POC) diagnostics, which could be integrated with nucleic acid-based tests and isothermal amplification experiments, enabling rapid sample-to-answer assays for field testing. Among the isothermal amplification experiments, loop-mediated isothermal amplification (LAMP), an extremely sensitive nucleic acid test, specifically identifies ultralow concentrations of DNA/RNA from practical samples for diagnosing diseases. We thus mainly focus on the paper device-based LAMP assay for the rapid infectious disease diagnosis, foodborne pathogen analysis, veterinary diagnosis, plant diagnosis, and environmental public health evaluation. We also outlined progress on paper microfluidic devices for drug delivery. The paper concludes with a discussion on the challenges of this technology and our insights into how to advance science and technology towards the development of fully functional paper devices in diagnostics and drug delivery.
纸是一种常见的材料，有望用于构建用于生物医学应用的诊断和药物输送的微流控芯片（纸上实验室）。在过去的十年中，基于纸张的微流控技术的广泛研究在生物医学诊断、食品安全、环境卫生、药物筛选和输送等领域积累了大量的科学出版物。本综述重点介绍了基于纸张的微流控技术的最新进展，重点介绍了该技术平台的设计、优化和应用，特别是在医学诊断和药物输送方面。新的进展集中在用于即时护理（POC）诊断的纸张设备的工程设计上，该设备可以与基于核酸的测试和等温扩增实验集成，从而实现快速的样本到答案分析，用于现场测试。在等温扩增实验中，环介导等温扩增（LAMP）是一种极其灵敏的核酸测试，可以特异性地从实际样本中识别出超低浓度的 DNA/RNA，用于诊断疾病。因此，我们主要关注基于纸张设备的 LAMP 检测方法，用于快速传染病诊断、食源性病原体分析、兽医诊断、植物诊断和环境公共卫生评估。我们还概述了用于药物输送的纸张微流控设备的进展。本文最后讨论了这项技术的挑战以及我们对如何推进科学技术以开发用于诊断和药物输送的全功能纸张设备的看法。
