Translational Stem Cell Biology Branch, National Heart, Lung, and Blood Institute, Bethesda, MD, USA.
Br J Haematol. 2021 Mar;192(5):819-831. doi: 10.1111/bjh.17175. Epub 2020 Nov 20.
Recent advances in high-throughput genomics have enabled the direct tracking of outputs from many cell types, greatly accelerating the study of developmental processes and tissue regeneration. The capacity for long-term self-renewal with multilineage differentiation potential characterises the cellular dynamics of a special set of developmental states that are critical for maintaining homeostasis. In haematopoiesis, the archetypal model for development, lineage-tracing experiments have elucidated the roles of haematopoietic stem cells to ongoing blood production and the importance of long-lived immune cells to immunological memory. An understanding of the biology and clonal dynamics of these cellular fates and states can provide clues to the response of haematopoiesis to ageing, the process of malignant transformation, and are key to designing more efficacious and durable clinical gene and cellular therapies.
高通量基因组学的最新进展使我们能够直接追踪多种细胞类型的输出，极大地加速了对发育过程和组织再生的研究。具有多谱系分化潜能的长期自我更新能力是维持内稳态的一组特殊发育状态的细胞动力学特征。在造血中，作为典型的发育模型，谱系追踪实验阐明了造血干细胞在持续造血中的作用，以及长寿免疫细胞对免疫记忆的重要性。了解这些细胞命运和状态的生物学和克隆动力学可以为理解造血对衰老、恶性转化过程的反应提供线索，也是设计更有效和更持久的临床基因和细胞治疗的关键。
