Weng Ching-Fu, Huang Chi-Jung, Wu Mei-Hsuan, Lee Henry Hsin-Chung, Ling Thai-Yen
Division of Pulmonary Medicine, Department of Internal Medicine, Hsinchu Cathay General Hospital, Hsinchu 300, Taiwan.
Department and Graduate Institute of Pharmacology, National Taiwan University, Taipei 100, Taiwan.
J Clin Med. 2020 Nov 18;9(11):3693. doi: 10.3390/jcm9113693.
Coxsackievirus/adenovirus receptors (CARs) and desmoglein-2 (DSG2) are similar molecules to adenovirus-based vectors in the cell membrane. They have been found to be associated with lung epithelial cell tumorigenesis and can be useful markers in predicting survival outcome in lung adenocarcinoma (LUAD).
A gene ontology enrichment analysis disclosed that DSG2 was highly correlated with CAR. Survival analysis was then performed on 262 samples from the Cancer Genome Atlas, forming "Stage 1A" or "Stage 1B". We therefore analyzed a tissue microarray (TMA) comprised of 108 lung samples and an immunohistochemical assay. Computer counting software was used to calculate the H-score of the immune intensity. Cox regression and Kaplan-Meier analyses were used to determine the prognostic value.
and genes are highly co-expressed in early stage LUAD and associated with significantly poorer survival ( = 0.0046). TMA also showed that CAR/DSG2 expressions were altered in lung cancer tissue. CAR in the TMA was correlated with proliferation, apoptosis, and epithelial-mesenchymal transition (EMT), while DSG2 was associated with proliferation only. The Kaplan-Meier survival analysis revealed that CAR, DSG2, or a co-expression of CAR/DSG2 was associated with poorer overall survival.
The co-expression of CAR/DSG2 predicted a worse overall survival in LUAD. CAR combined with DSG2 expression can predict prognosis.
柯萨奇病毒/腺病毒受体（CARs）和桥粒芯糖蛋白-2（DSG2）是细胞膜中与腺病毒载体相似的分子。它们已被发现与肺上皮细胞肿瘤发生有关，并且在预测肺腺癌（LUAD）的生存结果方面可能是有用的标志物。
基因本体富集分析显示DSG2与CAR高度相关。然后对来自癌症基因组图谱的262个样本进行生存分析，这些样本分为“1A期”或“1B期”。因此，我们分析了一个由108个肺样本组成的组织微阵列（TMA）并进行了免疫组织化学检测。使用计算机计数软件计算免疫强度的H评分。采用Cox回归和Kaplan-Meier分析来确定预后价值。
和 基因在早期LUAD中高度共表达，并且与显著较差的生存率相关（ = 0.0046）。TMA还显示肺癌组织中CAR/DSG2表达发生改变。TMA中的CAR与增殖、凋亡和上皮-间质转化（EMT）相关，而DSG2仅与增殖相关。Kaplan-Meier生存分析显示，CAR、DSG2或CAR/DSG2的共表达与较差的总生存率相关。
CAR/DSG2的共表达预示LUAD患者的总生存率较差。CAR与DSG2表达相结合可预测预后。
