Cell Cycle. 2020 Dec;19(23):3260-3276. doi: 10.1080/15384101.2020.1839697. Epub 2020 Nov 23.
Non-small cell lung cancer (NSCLC) is a leading cause of cancer death in both men and women. microRNAs (miRs) can exert important functions in cancer development. However, the role of miR-877 in NSCLC as it relates to tartrate resistant acid phosphatase 5 (ACP5) is unknown. For this study, the gain-and-loss-of-function experiments were performed to explore the effects of miR-877 and ACP5 on NSCLC. miR-877 expression in LC and paracancerous tissues, lung epithelial cell line and NSCLC cell lines was detected, and the association between miR-877 expression and clinical features of LC patients was analyzed. The levels of ACP5, epithelial-mesenchymal transition (EMT) markers and apoptosis-related proteins were measured. experiments were conducted for further validation. Consequently, we found that miR-877 expression was lowered in LC tissues and cell lines, and correlated with clinical stage, differentiation, lymph node metastasis and prognosis of NSCLC patients. Additionally, miR-877 was determined to inhibit ACP5 activity, and miR-877 downregulated the PI3K/AKT pathway by silencing ACP5. Furthermore, overexpression of miR-877 inhibited the viability, migration, invasion and EMT of NSCLC cells, but promoted cell apoptosis. In conclusion, miR-877 overexpression inhibited malignant biological behaviors of NSCLC cells by downregulating ACP5 and inactivating the PI3K/AKT pathway.
非小细胞肺癌（NSCLC）是男性和女性癌症死亡的主要原因。microRNAs（miRs）可以在癌症发展中发挥重要作用。然而，miR-877 在 NSCLC 中与抗酒石酸酸性磷酸酶 5（ACP5）的关系尚不清楚。在这项研究中，进行了增益和损耗功能实验，以探讨 miR-877 和 ACP5 对 NSCLC 的影响。检测了 LC 和癌旁组织、肺上皮细胞系和 NSCLC 细胞系中 miR-877 的表达，并分析了 miR-877 表达与 LC 患者临床特征的相关性。测量了 ACP5、上皮-间充质转化（EMT）标志物和凋亡相关蛋白的水平。进行了进一步的验证实验。结果表明，miR-877 在 LC 组织和细胞系中的表达降低，与 NSCLC 患者的临床分期、分化、淋巴结转移和预后相关。此外，miR-877 被确定可以抑制 ACP5 的活性，并且通过沉默 ACP5 下调了 PI3K/AKT 通路。此外，miR-877 的过表达抑制了 NSCLC 细胞的活力、迁移、侵袭和 EMT，但促进了细胞凋亡。总之，miR-877 的过表达通过下调 ACP5 和使 PI3K/AKT 通路失活来抑制 NSCLC 细胞的恶性生物学行为。
