Department of Food Science and Technology, Oregon State University, Corvallis 97330.
Protein Research Center, Agropur, Le Sueur, MN 56058.
J Dairy Sci. 2020 Dec;103(12):11079-11085. doi: 10.3168/jds.2020-18687. Epub 2020 Oct 23.
The main objective of this work was to develop an analytical method that can be used in a dairy manufacturing facility for the quantitation of phospholipids in dairy products. Total lipids from a dairy matrix were obtained first by Folch extraction. The total lipid extract was then applied to a silica gel-based solid-phase extraction column, and triglycerides and other nonpolar lipids were separated from the phospholipids and sphingolipids. Quantitation was performed by hydrophilic interaction HPLC coupled to evaporative light-scattering detection using a quaternary separation method. The method was validated using a commercial whey protein phospholipid concentrate and was used to analyze phospholipid and sphingolipid composition in buttermilk, whey protein concentrate, whey protein phospholipid concentrate, and several other dairy ingredients. This method was sensitive and reproducible and can be used in the dairy industry as a research tool to develop new value-added dairy phospholipid products, then later as a standard protocol for quality assurance analysis of current and future products.
本工作的主要目的是开发一种分析方法，可用于乳制品制造工厂中乳制品中磷脂的定量分析。首先通过 Folch 提取法从乳制品基质中获得总脂质。然后将总脂质提取物应用于基于硅胶的固相萃取柱，将甘油三酯和其他非极性脂质与磷脂和鞘脂分离。通过亲水相互作用 HPLC 结合蒸发光散射检测进行定量分析，采用四元分离方法。该方法使用商业乳清蛋白磷脂浓缩物进行验证，并用于分析酸乳、乳清蛋白浓缩物、乳清蛋白磷脂浓缩物和其他几种乳制品成分中的磷脂和鞘脂组成。该方法灵敏且重现性好，可在乳制品行业中用作开发新的有附加值的乳制品磷脂产品的研究工具，然后作为现有和未来产品质量保证分析的标准方案。
