Department of Food Science and Technology, Oregon State University, Corvallis, OR 97330, United States.
Department of Food Science and Technology, Oregon State University, Corvallis, OR 97330, United States; Department of Chemistry, Oregon State University, Corvallis, OR 97330, United States.
Food Chem. 2022 Apr 16;374:131495. doi: 10.1016/j.foodchem.2021.131495. Epub 2021 Oct 28.
Bioactive polar lipids in the milk fat globular membrane can be recovered and enriched during whey protein processing into a co-product called whey protein phospholipid concentrate (WPPC). A food-grade solvent successfully extracted polar lipids from powdered dairy products, and lipids can be fractionated under temperature-induced crystallization. This study investigates the specific lipid species present in ethanol extracted lipid residues from commercially available WPPC using a UPLC-Q-TOF-MS/MS lipidomics method. In general, sphingomyelins and phosphatidylcholines were retained in the polar lipid enriched fraction. Sphingomyelin was found to be a rich source of long chain fatty acids. Several glycosphingolipids, glucosyl-, galactosyl-, lactosyl-, and galabiosylceramide, were also detected in WPPC; these species were observed to crystallize away from other polar lipids during fractionation. Correlation analysis supported the claim that majority of polar lipids recovered in a total lipid extract using ethanol were retained in a polar lipid enriched residue after fractional crystallization.
在乳清蛋白加工过程中，可以从乳脂肪球膜中回收和富集具有生物活性的极性脂，并得到一种称为乳清蛋白磷酯浓缩物（WPPC）的副产物。一种食品级溶剂可以成功地从粉状乳制品中提取极性脂，并且可以在温度诱导结晶下对脂类进行分级。本研究使用 UPLC-Q-TOF-MS/MS 脂质组学方法，研究了从市售 WPPC 乙醇提取脂质残渣中存在的特定脂质种类。一般来说，在富含极性脂的部分中保留了鞘磷脂和磷脂酰胆碱。发现鞘磷脂是长链脂肪酸的丰富来源。在 WPPC 中还检测到几种糖鞘脂，即葡萄糖基、半乳糖基、乳糖基和半乳糖基神经酰胺；这些物质在分级分离过程中被观察到与其他极性脂分离结晶。相关分析支持以下观点，即使用乙醇从总脂质提取物中回收的大多数极性脂在分级结晶后保留在富含极性脂的残渣中。
