Avalli Andrea, Contarini Giovanna
CRA-lstituto Sperimentale Lattiero Caseario, via A. Lombardo 11, 26900 Lodi, Italy.
J Chromatogr A. 2005 Apr 15;1071(1-2):185-90. doi: 10.1016/j.chroma.2005.01.072.
The aim of this work was to evaluate the performance of different methods for both milk lipid extraction and phospholipids separation. As far as the lipid extraction procedure is concerned, the Folch method showed a higher phospholipid recovery with respect to the Rose-Gottlieb method. Different SPE cartridges and solvent phases were tested to carry out the separation of phospholipids from fat. The yield of extraction was evaluated by isolating phospholipids from both milk fat and synthetic fat; Standard Addition Method was applied as well. The isolation of the phospholipids by SPE silica column and subsequent analysis by HPLC/ELSD was shown to be an accurate and reproducible analytical method for the determination of phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine and sphingomyelin in milk fat extracted by Folch method.
这项工作的目的是评估不同的乳脂提取方法和磷脂分离方法的性能。就脂质提取程序而言，与罗斯 - 哥特利布方法相比，福尔克方法显示出更高的磷脂回收率。测试了不同的固相萃取（SPE）柱和溶剂相，以从脂肪中分离磷脂。通过从乳脂肪和合成脂肪中分离磷脂来评估提取产率；同时也应用了标准加入法。结果表明，通过SPE硅胶柱分离磷脂并随后通过高效液相色谱/蒸发光散射检测器（HPLC/ELSD）进行分析，是一种准确且可重复的分析方法，用于测定通过福尔克方法提取的乳脂肪中的磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰胆碱和鞘磷脂。
