Blaber M, Isackson P J, Bradshaw R A
Department of Biological Chemistry, California College of Medicine, University of California, Irvine 92717.
Biochemistry. 1987 Oct 20;26(21):6742-9. doi: 10.1021/bi00395a025.
The complete cDNA sequence of the major epidermal growth factor binding protein (EGF-BP), isolated from the mouse submandibular gland, has been determined. Oligonucleotide probes complementary to unique, nonconserved, regions of homogeneous preparations of EGF-BP were used to identify the correct cDNA clone from a male mouse submandibular gland cDNA library. The nucleotide sequence codes for a glandular kallikrein that is the main arginine esterase complexed with epidermal growth factor. The mRNA coding for this EGF-BP is estimated at 0.24% of the total mRNA of the adult male mouse submandibular gland, thus representing an abundant member of the kallikrein family in this tissue. In addition, the cDNA sequence defines a putative transcription start site. The reported cDNA sequence is clearly different from, and not an allelic form of, a previously reported cDNA sequence for EGF-BP. The present work reconciles conflicting information in the literature regarding the identity of EGF-BP.
已确定从小鼠颌下腺分离出的主要表皮生长因子结合蛋白（EGF - BP）的完整cDNA序列。与EGF - BP同质制剂独特的、非保守区域互补的寡核苷酸探针，用于从雄性小鼠颌下腺cDNA文库中鉴定正确的cDNA克隆。该核苷酸序列编码一种腺体激肽释放酶，它是与表皮生长因子复合的主要精氨酸酯酶。编码这种EGF - BP的mRNA估计占成年雄性小鼠颌下腺总mRNA的0.24%，因此是该组织中激肽释放酶家族的丰富成员之一。此外，cDNA序列确定了一个推定的转录起始位点。报道的cDNA序列与先前报道的EGF - BP的cDNA序列明显不同，且不是其等位基因形式。本研究调和了文献中关于EGF - BP身份的相互矛盾的信息。
