Alonso Maria Augusta, Silva Luciano Andrade, Affonso Fernanda Jordão, Lemes Kleber Menegon, Celeghini Eneiva Carla Carvalho, Lançoni Renata, Carvalho Henrique Fulanetti, de Arruda Rubens Paes
Laboratory of Semen Biotechnology and Andrology, Department of Animal Reproduction, School of Veterinary Medicine and Animal Science, University of Sao Paulo (USP), Pirassununga, São Paulo, Brazil.
Laboratory of Theriogenology Dr. O.J. Ginther, Department of Veterinary Medicine, School of Animal Sciences and Food Engineering, University of Sao Paulo, Pirassununga, São Paulo, Brazil.
Anim Reprod. 2019 Oct 24;16(2):317-327. doi: 10.21451/1984-3143-AR2018-0103.
Establishment of pregnancy after embryo transfer is the ultimate goal of an embryo transfer program and increasing pregnancy rates and reducing pregnancy loss are mandatory. The utilization of treatments to improve conception rates in recipient mares has been the focus of several research groups over the last years and the results are controversial. Some studies using human chorionic gonadotrophin (hCG) found promising results. Our hypothesis was that hCG administration would cause an additional stimulation on luteal function, uterine and luteal vascularization and progesterone concentration, and the mares would have increased uterine and cervix tone. Therefore, in the present study the effects of hCG administration to induce ovulation, on day 0 (day of ovulation) or day 5 postovulation were evaluated on corpus luteum characteristics, reproductive tract vascularization, and serum progesterone concentration from ovulation until day 15 postovulation. Groups were: G1: (control) - no hCG; G2: 2500 IU of hCG to induce ovulation when a follicle greater than 35mm and uterine edema were detected; G3: 2500 IU hCG on day 0; G4: 2500 IU hCG on day 5 postovulation. Twelve mares were randomly assigned to each group, during consecutive cycles, in a Latin Square experimental design, in a total of 48 cycles. Doppler ultrasound evaluations were performed daily from day 0 until day 15 postovulation, including mesometrial vascularity, endometrial vascularity and corpus luteum vascularity. Blood samples were collected for serum progesterone concentration. Data was analyzed using the Proc Glimmix SAS Procedure for nonparametric variables and Proc Mixed for parametric parameters. There was no treatment effect for all variables studied (P > 0.05). Characteristics were only affected by day (P < 0.05). It can be concluded that hCG administration at the time points suggested in the current study did not alter the characteristics evaluated.
胚胎移植后成功妊娠是胚胎移植计划的最终目标，提高妊娠率和减少妊娠丢失是必不可少的。在过去几年中，利用各种治疗方法提高受体母马的受孕率一直是多个研究小组关注的焦点，但其结果存在争议。一些使用人绒毛膜促性腺激素（hCG）的研究取得了有前景的结果。我们的假设是，给予hCG会对黄体功能、子宫和黄体血管生成以及孕酮浓度产生额外刺激，并且母马的子宫和宫颈张力会增加。因此，在本研究中，评估了在排卵日（第0天）或排卵后第5天给予hCG诱导排卵对黄体特征、生殖道血管生成以及从排卵到排卵后第15天血清孕酮浓度的影响。分组如下：G1：（对照组） - 不给予hCG；G2：当检测到卵泡大于35mm且子宫出现水肿时，给予2500IU hCG诱导排卵；G3：在第0天给予2500IU hCG；G4：在排卵后第5天给予2500IU hCG。采用拉丁方实验设计，在连续周期中，将12匹母马随机分配到每组，共进行48个周期。从第0天到排卵后第15天每天进行多普勒超声评估，包括子宫系膜血管、子宫内膜血管和黄体血管。采集血样测定血清孕酮浓度。使用Proc Glimmix SAS程序分析非参数变量数据，使用Proc Mixed分析参数参数数据。所有研究变量均未发现治疗效果（P>0.05）。各特征仅受天数影响（P<0.05）。可以得出结论，在本研究建议的时间点给予hCG不会改变所评估的特征。
