Nasir Abdul, Ashok Somasundar, Shim Jeung Yeop, Park Sunghoon, Yoo Tae Hyeon
Department of Molecular Science and Technology, Ajou University, Suwon, South Korea.
Bio R&D Center, Noroo Holdings Co., Ltd., Suwon, South Korea.
Front Bioeng Biotechnol. 2020 Nov 5;8:500867. doi: 10.3389/fbioe.2020.500867. eCollection 2020.
Coenzyme B-dependent glycerol dehydratase (GDHt) catalyzes the dehydration reaction of glycerol in the presence of adenosylcobalamin to yield 3-hydroxypropanal (3-HPA), which can be converted biologically to versatile platform chemicals such as 1,3-propanediol and 3-hydroxypropionic acid. Owing to the increased demand for biofuels, developing biological processes based on glycerol, which is a byproduct of biodiesel production, has attracted considerable attention recently. In this review, we will provide updates on the current understanding of the catalytic mechanism and structure of coenzyme B-dependent GDHt, and then summarize the results of engineering attempts, with perspectives on future directions in its engineering.
辅酶B依赖型甘油脱水酶（GDHt）在腺苷钴胺素存在的情况下催化甘油的脱水反应，生成3-羟基丙醛（3-HPA），3-羟基丙醛可通过生物转化生成多种平台化学品，如1,3-丙二醇和3-羟基丙酸。由于对生物燃料的需求增加，基于甘油（生物柴油生产的副产品）开发生物工艺最近引起了相当大的关注。在这篇综述中，我们将介绍关于辅酶B依赖型GDHt催化机制和结构的当前认识的最新进展，然后总结工程尝试的结果，并展望其工程领域的未来发展方向。
