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通过基因工程将甘油转化为3-羟基丙酸

Conversion of Glycerol to 3-Hydroxypropanoic Acid by Genetically Engineered .

作者信息

Kalantari Aida, Chen Tao, Ji Boyang, Stancik Ivan A, Ravikumar Vaishnavi, Franjevic Damjan, Saulou-Bérion Claire, Goelzer Anne, Mijakovic Ivan

机构信息

Systems and Synthetic Biology Division, Department of Biology and Biological Engineering, Chalmers University of TechnologyGothenburg, Sweden.

Chaire Agro-Biotechnologies Industrielles, AgroParisTechReims, France.

出版信息

Front Microbiol. 2017 Apr 18;8:638. doi: 10.3389/fmicb.2017.00638. eCollection 2017.

DOI:10.3389/fmicb.2017.00638
PMID:28458661
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5394112/
Abstract

3-Hydroxypropanoic acid (3-HP) is an important biomass-derivable platform chemical that can be converted into a number of industrially relevant compounds. There have been several attempts to produce 3-HP from renewable sources in cell factories, focusing mainly on , and . Despite the significant progress made in this field, commercially exploitable large-scale production of 3-HP in microbial strains has still not been achieved. In this study, we investigated the potential of as a microbial platform for bioconversion of glycerol into 3-HP. Our recombinant strains overexpress the two-step heterologous pathway containing glycerol dehydratase and aldehyde dehydrogenase from . Genetic engineering, driven by optimization, and optimization of cultivation conditions resulted in a 3-HP titer of 10 g/L, in a standard batch cultivation. Our findings provide the first report of successful introduction of the biosynthetic pathway for conversion of glycerol into 3-HP in . With this relatively high titer in batch, and the robustness of in high density fermentation conditions, we expect that our production strains may constitute a solid basis for commercial production of 3-HP.

摘要

3-羟基丙酸（3-HP）是一种重要的可从生物质中获取的平台化学品，可转化为多种与工业相关的化合物。已经有若干尝试在细胞工厂中从可再生资源生产3-HP，主要集中在[此处原文缺失相关内容]。尽管该领域取得了显著进展，但微生物菌株中可商业开发的大规模3-HP生产仍未实现。在本研究中，我们研究了[此处原文缺失相关内容]作为将甘油生物转化为3-HP的微生物平台的潜力。我们的重组[此处原文缺失相关内容]菌株过表达了来自[此处原文缺失相关内容]的包含甘油脱水酶和醛脱氢酶的两步异源途径。由[此处原文缺失相关内容]优化驱动的基因工程以及培养条件的优化在标准分批培养中产生了10 g/L的3-HP滴度。我们的研究结果首次报道了在[此处原文缺失相关内容]中成功引入将甘油转化为3-HP的生物合成途径。鉴于分批培养中有相对较高的滴度以及[此处原文缺失相关内容]在高密度发酵条件下的稳健性，我们预计我们的生产菌株可能构成3-HP商业生产的坚实基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/1e6dcca18908/fmicb-08-00638-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/ba4ba9f1aa70/fmicb-08-00638-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/41dda08f667a/fmicb-08-00638-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/f6f810068750/fmicb-08-00638-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/f70535e350d0/fmicb-08-00638-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/039bec69ae18/fmicb-08-00638-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/ea28d88be282/fmicb-08-00638-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/1e6dcca18908/fmicb-08-00638-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/ba4ba9f1aa70/fmicb-08-00638-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/41dda08f667a/fmicb-08-00638-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/f6f810068750/fmicb-08-00638-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/f70535e350d0/fmicb-08-00638-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/039bec69ae18/fmicb-08-00638-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/ea28d88be282/fmicb-08-00638-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adda/5394112/1e6dcca18908/fmicb-08-00638-g007.jpg

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