Park Yun Ji, Baek Seung-A, Kim Jae Kwang, Park Sang Un
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Republic of Korea.
ACS Omega. 2020 Nov 5;5(45):29312-29324. doi: 10.1021/acsomega.0c04001. eCollection 2020 Nov 17.
Fisch ex DC belongs to the Compositae family and has been used as a folk remedy source in Asian countries because of its health-promoting properties. It is known that contains flavonoids, furans, long-chain alcohols, sterols, and volatile oils. Nevertheless, the molecular mechanism of secondary metabolite biosynthesis remains poorly understood. Therefore, transcriptome analysis and metabolic profiling were performed using different parts of to investigate phenylpropanoid metabolism. Based on the BLASTX search results, we identified 29 orthologs of enzymes responsible for phenylpropanoid biosynthesis. Additionally, 75 metabolites were identified in . Most of the flavonoid biosynthetic genes were significantly expressed ranging from 2.6- to 500-fold higher in the flowers than those in the leaves. Correspondently, the total content of flavonols was 21-fold higher in the flowers than in the roots. However, the total level of flavones showed 58-fold higher amounts in the leaves than in the flowers. Additionally, the total content of flavanols was 19-fold higher in the leaves than in the roots. The results of this study provide transcriptomic and metabolic information to elucidate the tissue-specific phenylpropanoid metabolism of .
紫花飞蓬属于菊科植物，因其具有促进健康的特性，在亚洲国家一直被用作民间药物来源。已知其含有黄酮类化合物、呋喃类、长链醇、甾醇和挥发油。然而，其次生代谢产物生物合成的分子机制仍知之甚少。因此，利用紫花飞蓬的不同部位进行转录组分析和代谢谱分析，以研究苯丙烷类代谢。基于BLASTX搜索结果，我们鉴定出29个负责苯丙烷类生物合成的酶的直系同源物。此外，在紫花飞蓬中鉴定出75种代谢产物。大多数黄酮类生物合成基因在花中的表达显著高于叶，其表达量高出2.6至500倍。相应地，花中黄酮醇的总含量比根中高21倍。然而，叶中黄酮的总含量比花中高58倍。此外，叶中黄烷醇的总含量比根中高19倍。本研究结果提供了转录组和代谢信息，以阐明紫花飞蓬组织特异性苯丙烷类代谢。
