Full-Length Transcriptome Sequencing and Comparative Transcriptomic Analyses Provide Comprehensive Insight into Molecular Mechanisms of Flavonoid Metabolites Biosynthesis in .

L. is a commonly consumed plant in China, known for its medicinal and nutritional benefits. This study focuses on the medicinal properties influenced by flavonoid metabolites, which vary during flower development. Utilizing full-length transcriptome sequencing on flowers, we observed changes in gene expression levels as the flowers progressed through growth stages. During stages S1 and S2, key genes related to flavonoid synthesis (, , , , etc.) exhibited heightened expression. A weighted gene co-expression network analysis (WGCNA) identified regulatory genes (, , ) potentially involved in the regulatory network with flavonoid biosynthesis-related genes. Our findings propose a regulatory mechanism for flavonoid synthesis in flowers, elucidating the genetic underpinnings of this process. The identified candidate genes present opportunities for genetic enhancements in , offering insights into potential applications for improving its medicinal attributes.