Manciula Lavinia-Gianina, Jeican Ionut Isaia, Tudoran Lucian Barbu, Albu Silviu
2 Department of Otolaryngology, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Department of Head and Neck Surgery and Otorhinolaryngology, University Clinical Hospital of Railway Company, Iuliu Hatieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania. Department of Anatomy and Embryology, Iuliu Haţieganu University of Medicine and Pharmacy Cluj-Napoca.
Med Pharm Rep. 2020 Oct;93(4):374-383. doi: 10.15386/mpr-1691. Epub 2020 Oct 25.
The aim of the present study is to evaluate the presence of biofilms in patients with chronic rhinosinusitis (CRS), with or without nasal polyps, and their relationship to eosinophils and plasma cells. We compared the results with those obtained in nonCRS patients.
A total of 50 patients were included in the study, 30 CRSwNP patients, 10 CRSsNP cases and 10 control patients who were operated for deviated septum. Biofilm detection was performed by means of H&E staining and SEM. Eosinophil and plasma cell values were recorded and compared between groups.
Biofilms were identified in 30 patients (60%), 76.6% (23 out of 30) of the CRSwNP patients, 70% (7 out of 10) of the CRSsNP patients and none of the septoplasty patients. Eosinophil and plasma cell values were more elevated in CRS patients, being strongly correlated to biofilm presence and nasal polyposis.
Biofilm presence was demonstrated in many of the CRS patients, with no evidence in the control cases. Our study findings indicate that inflammatory cell counts are higher in patients with CRS compared to controls, but also more elevated in patients with polyposis. In biofilm-positive patients, eosinophil and plasma cell counts were greater than those in patients without biofilms, demonstrating the proinflammatory action of the biofilm in the sino-nasal pathology.
本研究的目的是评估慢性鼻-鼻窦炎（CRS）患者（伴或不伴鼻息肉）生物膜的存在情况及其与嗜酸性粒细胞和浆细胞的关系。我们将结果与非CRS患者的结果进行了比较。
本研究共纳入50例患者，30例伴鼻息肉的慢性鼻-鼻窦炎（CRSwNP）患者、10例不伴鼻息肉的慢性鼻-鼻窦炎（CRSsNP）患者以及10例因鼻中隔偏曲接受手术的对照患者。通过苏木精-伊红（H&E）染色和扫描电子显微镜（SEM）进行生物膜检测。记录并比较各组嗜酸性粒细胞和浆细胞的值。
30例患者（60%）检测到生物膜，其中CRSwNP患者中有76.6%（30例中的23例）、CRSsNP患者中有70%（10例中的7例）检测到生物膜，而鼻中隔成形术患者中未检测到生物膜。CRS患者的嗜酸性粒细胞和浆细胞值更高，且与生物膜的存在及鼻息肉密切相关。
许多CRS患者存在生物膜，对照病例中未发现相关证据。我们的研究结果表明，与对照组相比，CRS患者的炎症细胞计数更高，鼻息肉患者的炎症细胞计数更高。在生物膜阳性患者中，嗜酸性粒细胞和浆细胞计数高于无生物膜患者，表明生物膜在鼻-鼻窦病理过程中具有促炎作用。
