Novotny M J, Turrentine M A, Johnson G S, Adams H R
Department of Veterinary Biomedical Sciences, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Circ Shock. 1987;23(3):205-13.
Pentobarbital-anesthetized beagles were infused with physiologic saline (control dogs) or E. coli endotoxin (1.5 mg/kg i.v.) for 1 min. The plasma von Willebrand factor antigen (vWf:Ag) concentration, a potential index of endothelial damage, was monitored before and for 4 h after endotoxin challenge. The plasma vWf:Ag concentration increased only slightly in the control dogs (n = 6); whereas, in dogs infused with endotoxin (n = 20), the vWf:Ag concentration increased progressively to 2.1 times prechallenge values in 4 h. In addition to endotoxin, some of these dogs were treated with free-radical scavengers; allopurinol (n = 3), allopurinol plus superoxide dismutase and catalase (n = 6), or deferoxamine (n = 5). The free-radical scavengers did not prevent endotoxin-induced increases in vWf:Ag concentration.
对戊巴比妥麻醉的比格犬静脉输注生理盐水（对照犬）或大肠杆菌内毒素（1.5毫克/千克静脉注射）1分钟。监测血浆血管性血友病因子抗原（vWf:Ag）浓度，这是内皮损伤的一个潜在指标，在内毒素攻击前及攻击后4小时进行监测。对照犬（n = 6）的血浆vWf:Ag浓度仅略有增加；而在内毒素输注的犬（n = 20）中，vWf:Ag浓度在4小时内逐渐增加至攻击前值的2.1倍。除了内毒素外，其中一些犬还用自由基清除剂进行了治疗；别嘌醇（n = 3）、别嘌醇加超氧化物歧化酶和过氧化氢酶（n = 6）或去铁胺（n = 5）。自由基清除剂未能阻止内毒素诱导的vWf:Ag浓度升高。
