Kraus K H, Turrentine M A, Johnson G S
Department of Veterinary Medicine and Surgery, University of Missouri 65211.
Am J Vet Res. 1987 Sep;48(9):1376-9.
Eight adult male Beagles were given 1 microgram of 1-deamino-8-D-arginine vasopressin (DDAVP)/kg of body weight, SC or by slow IV infusion on separate occasions. Both routes of administration induced highly significant increases (P less than 0.0001) in plasma concentrations of von Willebrand factor (vWf) antigen (Ag) and botrocetin cofactor (BCf) activity, an indication of platelet-associated vWf activity. In most instances, increases in plasma vWf:Ag and BCf values induced by SC injection were as large as or larger than those induced by slow IV infusion. With both routes of administration, BCf activity increased more than the vWf:Ag concentration, so that high BCf-to-vWf:Ag ratios were found in plasma after DDAVP administration. In plasma samples obtained before DDAVP administration, sodium dodecyl sulfate-electrophoresis resolved the vWf into a series of multimeric proteins with molecular weights similar to those of human vWf. Sodium dodecyl sulfate-electrophoresis of plasma samples obtained after DDAVP administration revealed mainly the larger molecules of vWf that were increased by DDAVP administration.
八只成年雄性比格犬在不同时间分别接受皮下注射或缓慢静脉输注1微克/千克体重的1-去氨基-8-D-精氨酸加压素（DDAVP）。两种给药途径均导致血管性血友病因子（vWf）抗原（Ag）和巴曲酶辅因子（BCf）活性的血浆浓度显著升高（P<0.0001），这表明血小板相关的vWf活性增强。在大多数情况下，皮下注射诱导的血浆vWf:Ag和BCf值升高幅度与缓慢静脉输注相当或更大。两种给药途径下，BCf活性的增加均超过vWf:Ag浓度的增加，因此在给予DDAVP后血浆中发现了高BCf与vWf:Ag比值。在给予DDAVP之前采集的血浆样本中，十二烷基硫酸钠电泳将vWf分解为一系列分子量与人vWf相似的多聚体蛋白。给予DDAVP后采集的血浆样本的十二烷基硫酸钠电泳显示，主要是DDAVP给药后增加的较大分子量的vWf分子。
